Hendry Cindy, Dionne Kim, Hedgepeth Annie, Carroll Karen, Parrish Nicole
The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA.
J Clin Microbiol. 2009 Apr;47(4):1206-8. doi: 10.1128/JCM.02097-08. Epub 2009 Feb 25.
Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time ( approximately 2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories.
快速检测分枝杆菌病至关重要。我们使用多种标本类型和分枝杆菌浓度,比较了两种市售金胺O染色剂。这种更快的染色剂能够实现一致的抗酸杆菌定量,且背景染色更少,染色过程所需时间更短(约2分钟)。这些结果表明,这种快速染色剂在临床实验室使用可能更具成本效益和效率。