Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, Belgium.
Virus Res. 2010 Sep;152(1-2):180-3. doi: 10.1016/j.virusres.2010.06.008. Epub 2010 Jun 22.
Statins, such as lovastatin, inhibit the cellular cholesterol biosynthesis. Addition of lovastatin to SK cells and subsequent infection with the alphaherpesvirus pseudorabies virus (PRV) did not affect the intracellular production of viral structural proteins but reduced virus titers. Addition of methyl-beta-cyclodextrin to deplete cholesterol from the viral envelope also resulted in reduced virus titers. Addition of exogenous cholesterol restored virus titers in both experimental assays. Further analysis showed that reducing cholesterol levels reduced both the infectivity of newly produced infectious virus and their stability, as assessed by determining virus titers immediately after treatment or upon storage of the virus at room temperature or frozen. This is the first report to demonstrate that cholesterol is involved in the stability of infectious alphaherpesvirus, and that treatment of host cells with statins reduces alphaherpesvirus titers. Hence, cholesterol is important for pseudorabies virus infectivity and stability.
他汀类药物，如洛伐他汀，可抑制细胞内胆固醇的生物合成。在 SK 细胞中添加洛伐他汀并随后感染α疱疹伪狂犬病病毒 (PRV) 不会影响病毒结构蛋白的细胞内产生，但会降低病毒滴度。添加甲基-β-环糊精从病毒包膜中耗尽胆固醇也会导致病毒滴度降低。添加外源性胆固醇可恢复两种实验检测中的病毒滴度。进一步的分析表明，降低胆固醇水平会降低新产生的传染性病毒的感染力及其稳定性，这可以通过在处理后立即测定病毒滴度或在室温或冷冻下储存病毒来评估。这是第一个证明胆固醇参与传染性α疱疹病毒稳定性的报告，并且用他汀类药物处理宿主细胞会降低α疱疹病毒的滴度。因此，胆固醇对伪狂犬病病毒的感染力和稳定性很重要。
