Desplanques Ann S, Nauwynck Hans J, Vercauteren Dries, Geens Tom, Favoreel Herman W
Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
Virology. 2008 Jul 5;376(2):339-45. doi: 10.1016/j.virol.2008.03.039. Epub 2008 May 8.
Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significantly reduces PRV entry. Cholesterol depletion did not reduce PRV attachment, but stalled virus particles at the plasma membrane before penetration of the cell. Cholesterol depletion results in destabilization of lipid raft microdomains in the plasma membrane, which have been shown before to be involved in efficient entry of different viruses. A significant fraction of PRV virions appears to localize juxtaposed to GM1, a lipid raft marker, during entry. Together, these data indicate that cholesterol and possibly cholesterol-rich lipid rafts may be important during PRV entry.
甲型疱疹病毒包括与人类和动物密切相关的病毒，包括单纯疱疹病毒、水痘-带状疱疹病毒和伪狂犬病病毒（PRV）。在此，我们使用甲基-β-环糊精和荧光标记的PRV，直接表明从宿主细胞质膜中耗尽胆固醇会显著减少PRV的进入。胆固醇耗尽并没有减少PRV的附着，但在病毒颗粒穿透细胞之前使其停滞在质膜上。胆固醇耗尽导致质膜中脂筏微结构域的不稳定，此前已表明脂筏微结构域参与不同病毒的有效进入。在进入过程中，相当一部分PRV病毒粒子似乎定位在与脂筏标记物GM1并列的位置。这些数据共同表明，胆固醇以及可能富含胆固醇的脂筏在PRV进入过程中可能很重要。
