Zhang J Q, Zhao M
Changhai Hospital, Second Military Medical College, Shanghai.
Zhong Xi Yi Jie He Za Zhi. 1991 Feb;11(2):105-6, 69-70.
Hyperthyroxinemia model was made by giving thyroid tablet suspension to Wistar rats and hypothyroxinemia model was made by thyroidectomy. We measured serum thyroid hormone levels by RIA and the parameters of triiodothyronine receptors in rat hepatic cell nucleus by radio-ligand binding assay: Maximal binding capacity (Bmax) and Dissociation constant (Kd). It is found that (1) Yin-tonics can lower serum thyroid hormone levels and Bmax of hepatic nuclear T3R of hyperthyroxinemia rat from 167.14 +/- 25.62 fmol/100 micrograms DNA to 98.98 +/- 15.24 fmol/100 micrograms DNA, P less than 0.001. (2) Both Yang-tonics I and II can raise serum thyroid hormone levels of hypothyroxinemia rats, but not Bmax of hepatic nuclear T3R. Yang-tonics I even lowers Bmax. All the Chinese herbs have no effect on the Kd of rat hepatic nuclear T3R. The results may have some value in studying the effects of Chinese medical drugs.
给Wistar大鼠喂服甲状腺片悬液制成甲状腺素血症模型，通过甲状腺切除术制成甲状腺素缺乏血症模型。我们用放射免疫分析法测定血清甲状腺激素水平，用放射性配体结合分析法测定大鼠肝细胞核中三碘甲状腺原氨酸受体的参数：最大结合容量（Bmax）和解离常数（Kd）。结果发现：（1）滋阴药可使甲状腺素血症大鼠血清甲状腺激素水平及肝细胞核T3R的Bmax从167.14±25.62fmol/100μg DNA降至98.98±15.24fmol/100μg DNA，P＜0.001。（2）补阳药I和补阳药II均可提高甲状腺素缺乏血症大鼠的血清甲状腺激素水平，但对肝细胞核T3R的Bmax无影响。补阳药I甚至使Bmax降低。所有中药对大鼠肝细胞核T3R的Kd均无影响。这些结果可能对研究中药的作用有一定价值。
