Department of Evolutionary Biology, Evolutionary Biology Centre, Uppsala University, Uppsala, Sweden.
Mol Phylogenet Evol. 2010 Oct;57(1):466-70. doi: 10.1016/j.ympev.2010.06.009. Epub 2010 Jun 25.
Classically, the mitochondrial genome is sequenced by a series of amplicons using conserved PCR primers. Here we show how shot-gun transcriptome sequencing can be used to obtain the complete set of protein-coding genes from the mtDNA of four passerine bird species. With these sequences, we address the still unresolved basal Passerida relationships (Aves: Passeriformes). Our analysis suggests a new hypothesis for the basal relationships of Passerida, namely a clade grouping Sylvioidea and Passeroidea, with Paridae and Muscicapidae as successive sister groups to this clade. This study demonstrates the usefulness of next-generation sequencing transcriptome sequencing for obtaining new mtDNA genomes.
经典方法是使用保守的 PCR 引物通过一系列扩增子来对线粒体基因组进行测序。在这里，我们展示了如何利用鸟枪法转录组测序从四种雀形目鸟类的 mtDNA 中获得完整的蛋白质编码基因集。利用这些序列，我们解决了尚未解决的雀形目基部关系（鸟类：雀形目）。我们的分析提出了一个新的雀形目基部关系的假说，即一个包含 Sylvioidea 和 Passeroidea 的分支，与 Paridae 和 Muscicapidae 作为这个分支的连续姐妹群。本研究证明了下一代测序转录组测序在获得新的 mtDNA 基因组方面的有用性。
