Zhang Huabin, Bai Yuze, Shi Xuejia, Sun Linxia, Wang Zhengfei, Wu Xiaobing
Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224001, Jiangsu Province, China.
Anhui Province Key Laboratory for Conservation and Exploitation of Biological Resource, College of Life Sciences, Anhui Normal University, Wuhu, China.
Genes Genomics. 2018 Feb;40(2):151-165. doi: 10.1007/s13258-017-0617-5. Epub 2017 Oct 14.
Passeriformes is the largest group within aves and the phylogenetic relationships between Passeriformes have caused major disagreement in ornithology. Particularly, the phylogenetic relationships between muscicapoidea and sylvioidea are complex, and their taxonomic boundaries have not been clearly defined. Our aim was to study the status of two bird species: Tarsiger cyanurus and Phoenicurus auroreus. Furthermore, we analyzed the phylogenetic relationships of Passeriformes. Complete mitochondrial DNA (mtDNA) sequences of both species were determined and the lengths were 16,803 (T. cyanurus) and 16,772 bp (P. auroreus), respectively. Thirteen protein-coding genes, 22 tRNA genes, two rRNA genes, and one control region were identified in these mtDNAs. The contents of A and T at the base compositions was significantly higher than the content of G and C, and this AT skew was positive, while the GC skew was negative. The monophyly of Passeriformes is divided into four major clades: Corvoidea, Sylvioidea, Passeroidea, and Musicicapoidea. Paridae should be separated from the superfamily Sylvioidea and placed within the superfamily Muscicapoidea. The family Muscicapidae and Corvida were paraphyly, while Carduelis and Emberiza were grouped as a sister taxon. The relationships between some species of the order passeriformes may remain difficult to resolve despite an effort to collect additional characters for phylogenetic analysis. Current research of avian phylogeny should focus on adding characters and taxa and use both effectively to obtain a better resolution for deeper and shallow nodes.
雀形目是鸟类中最大的类群，雀形目内部的系统发育关系在鸟类学中引起了重大分歧。特别是，鸫总科和莺总科之间的系统发育关系很复杂，它们的分类界限尚未明确界定。我们的目的是研究两种鸟类的分类地位：蓝喉歌鸲和红腹红尾鸲。此外，我们分析了雀形目的系统发育关系。测定了这两个物种的完整线粒体DNA（mtDNA）序列，长度分别为16,803（蓝喉歌鸲）和16,772 bp（红腹红尾鸲）。在这些线粒体DNA中鉴定出13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和1个控制区。碱基组成中A和T的含量显著高于G和C的含量，这种AT偏斜为正，而GC偏斜为负。雀形目的单系性分为四个主要分支：鸦总科、莺总科、雀总科和鸫总科。山雀科应从莺总科中分离出来，置于鸫总科内。鸫科和鸦小目的分类是并系的，而金翅雀属和鹀属被归为姐妹分类单元。尽管努力收集更多特征进行系统发育分析，但雀形目一些物种之间的关系可能仍然难以解决。当前鸟类系统发育研究应侧重于增加特征和分类单元，并有效地利用两者来获得更好的分辨率，以解决较深和较浅的节点问题。
