Department of Mechanical Engineering, Faculty of Engineering, The University of Hong Kong, Hong Kong.
Acta Biomater. 2010 Dec;6(12):4495-505. doi: 10.1016/j.actbio.2010.06.024. Epub 2010 Jun 30.
Bionanocomposites formed by combining biodegradable polymers and nanosized osteoconductive inorganic solids have been regarded as promising biomimetic systems which possess much improved structural and functional properties for bone tissue regeneration. In this study three-dimensional nanocomposite scaffolds based on calcium phosphate (Ca-P)/poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) and carbonated hydroxyapatite (CHAp)/poly(l-lactic acid) (PLLA) nanocomposite microspheres were successfully fabricated using selective laser sintering, which is a rapid prototyping technology. The sintered scaffolds had controlled material microstructure, totally interconnected porous structure and high porosity. The morphology and mechanical properties of Ca-P/PHBV and CHAp/PLLA nanocomposite scaffolds as well as PHBV and PLLA polymer scaffolds were studied. In vitro biological evaluation showed that SaOS-2 cells had high cell viability and normal morphology and phenotype after 3 and 7 days culture on all scaffolds. The incorporation of Ca-P nanoparticles significantly improved cell proliferation and alkaline phosphatase activity for Ca-P/PHBV scaffolds, whereas CHAp/PLLA nanocomposite scaffolds exhibited a similar level of cell response compared with PLLA polymer scaffolds. The nanocomposite scaffolds provide a biomimetic environment for osteoblastic cell attachment, proliferation and differentiation and have great potential for bone tissue engineering applications.
将可生物降解聚合物与纳米级骨传导无机固体结合形成的生物纳米复合材料被认为是有前途的仿生系统，它们具有改善的结构和功能特性，可用于骨组织再生。在这项研究中，使用选择性激光烧结成功制备了基于磷酸钙 (Ca-P)/聚(羟基丁酸酯-co-羟基戊酸酯) (PHBV) 和碳酸羟基磷灰石 (CHAp)/聚 (L-乳酸) (PLLA) 纳米复合材料微球的三维纳米复合材料支架，选择性激光烧结是一种快速原型制造技术。烧结支架具有受控的材料微观结构、完全连通的多孔结构和高孔隙率。研究了 Ca-P/PHBV 和 CHAp/PLLA 纳米复合材料支架以及 PHBV 和 PLLA 聚合物支架的形态和机械性能。体外生物学评估表明，SaOS-2 细胞在所有支架上培养 3 天和 7 天后具有高细胞活力和正常形态和表型。Ca-P 纳米粒子的掺入显著提高了 Ca-P/PHBV 支架的细胞增殖和碱性磷酸酶活性，而 CHAp/PLLA 纳米复合材料支架与 PLLA 聚合物支架相比表现出相似的细胞反应水平。纳米复合材料支架为成骨细胞的附着、增殖和分化提供了仿生环境，在骨组织工程应用中具有巨大的潜力。
