GEMSEC, Genetically Engineered Materials Science and Engineering Center, Department of Materials Science & Engineering, University of Washington, Seattle, 98195 WA, USA.
Acta Biomater. 2010 Dec;6(12):4634-41. doi: 10.1016/j.actbio.2010.06.004. Epub 2010 Jul 3.
Uncontrolled interactions between synthetic materials and human tissues are a major concern for implants and tissue engineering. The most successful approaches to circumvent this issue involve the modification of the implant or scaffold surfaces with various functional molecules, such as anti-fouling polymers or cell growth factors. To date, such techniques have relied on surface immobilization methods that are often applicable only to a limited range of materials and require the presence of specific functional groups, synthetic pathways or biologically hostile environments. In this study we have used peptide motifs that have been selected to bind to gold, platinum, glass and titanium to modify surfaces with poly(ethylene glycol) anti-fouling polymer and the integrin-binding RGD sequence. The peptides have several advantages over conventional molecular immobilization techniques; they require no biologically hostile environments to bind, are specific to their substrates and could be adapted to carry various active entities. We successfully imparted cell-resistant properties to gold and platinum surfaces using gold- and platinum-binding peptides, respectively, in conjunction with PEG. We also induced a several-fold increase in the number and spreading of fibroblast cells on glass and titanium surfaces using quartz and titanium-binding peptides in conjunction with the integrin ligand RGD. The results presented here indicate that control over the extent of cell-material interactions can be achieved by relatively simple and biocompatible surface modification procedures using inorganic binding peptides as linker molecules.
合成材料与人体组织之间的不可控相互作用是植入物和组织工程的主要关注点。规避这个问题最成功的方法是用各种功能分子（如抗污聚合物或细胞生长因子）来修饰植入物或支架表面。迄今为止，这些技术依赖于表面固定化方法，这些方法通常仅适用于有限范围的材料，并且需要存在特定的功能基团、合成途径或生物敌对环境。在这项研究中，我们使用了经过选择与金、铂、玻璃和钛结合的肽基序来修饰表面，使其具有聚乙二醇抗污聚合物和整合素结合的 RGD 序列。与传统的分子固定化技术相比，这些肽具有几个优势；它们不需要生物敌对环境就能结合，对其底物具有特异性，并且可以适应携带各种活性实体。我们成功地使用金结合肽和铂结合肽分别与 PEG 一起，赋予金和铂表面抗细胞特性。我们还使用石英结合肽和钛结合肽与整合素配体 RGD 一起，使玻璃和钛表面上的成纤维细胞数量和铺展增加了几倍。这里呈现的结果表明，可以通过使用无机结合肽作为连接分子的相对简单和生物相容的表面修饰程序来实现对细胞-材料相互作用程度的控制。
