Quesniaux V F
Sandoz Pharma AG, Basel, Switzerland.
Clin Biochem. 1991 Feb;24(1):37-42. doi: 10.1016/0009-9120(91)90147-7.
Monitoring blood levels of Cyclosporine (CsA) has been the basis for adjusting individual dosage regimens in the clinic. Radioimmunoassays using polyclonal antisera reacted with CsA and some CsA metabolites, leading to overestimation when compared with high-performance liquid chromatographic measurements of CsA. Monoclonal antibodies (mAbs) have the potential to discriminate between closely related molecules. MAbs with high affinity for CsA have been prepared and their fine-specificity characterized by cross-reactivity studies using a large series of CsA-derivatives. According to the known sites of metabolism on the CsA molecule and to its three-dimensional structure, it was possible to predict which mAb would be suitable for recognizing native Cs specifically.
监测环孢素（CsA）的血药浓度一直是临床上调整个体化给药方案的依据。使用多克隆抗血清的放射免疫分析与CsA及一些CsA代谢产物发生反应，与CsA的高效液相色谱测量结果相比会导致高估。单克隆抗体（mAb）有区分密切相关分子的潜力。已制备出对CsA具有高亲和力的单克隆抗体，并通过使用大量CsA衍生物的交叉反应研究对其精细特异性进行了表征。根据CsA分子上已知的代谢位点及其三维结构，可以预测哪种单克隆抗体适合特异性识别天然Cs。
