Alcántara Cristina, Peirotén Ngela, Ramón-Núñez Luís Andrés, Landete José María, Monedero Vicente, Zúñiga Manuel
Laboratorio de Bacterias Lcticas y Probiticos, Instituto de Agroqumica y Tecnologa de Alimentos (IATA-CSIC), Av. Agustn Escardino 7, 46980 Paterna, Spain.
Departamento de Tecnologa de Alimentos, National Institute for Agricultural and Food Research and Technology (INIA-CSIC), Carretera de La Corua Km 7.5, 28040 Madrid, Spain.
Int J Syst Evol Microbiol. 2025 Jan;75(1). doi: 10.1099/ijsem.0.006654.
A novel strain of the genus , named He02, was isolated from flowers of L. in a survey for lactic acid bacteria associated with wild and cultivated plants in the metropolitan area of Valencia, Spain. Partial 16S rRNA gene sequencing revealed a similarity of 99% to DSM 23037=Ryu1-2. Strain He02 cells are Gram-stain-positive, catalase-negative non-motile rods, usually occurring in pairs. Cells show a pale yellow pigmentation when pelleted. As , strain He02 utilized a narrow range of carbohydrates, namely, glucose and fructose, homofermentatively. However, genome sequencing and estimation of average nucleotide identity (ANI) revealed an ANI value of 87.44 with DSM 23037, the only strain sequenced to date. A value of 30.5% for digital DNA-DNA hybridization was estimated with the Type Strain Genome Server tool when He02 was compared with strain DSM 23037. These results indicate that strain He02 constitutes a novel species, for which the name sp. nov. with He02 (=CECT 31001=DSM 117324=CCM 9395) as type strain is proposed.
在对西班牙巴伦西亚大都市区野生和栽培植物相关乳酸菌的调查中，从 的花中分离出一种新的 属菌株，命名为 He02。16S rRNA 基因部分测序显示与 DSM 23037=Ryu1-2 的相似度为 99%。菌株 He02 细胞为革兰氏染色阳性、过氧化氢酶阴性的无运动性杆菌，通常成对出现。细胞沉淀时呈淡黄色。作为 ，菌株 He02 利用的碳水化合物范围较窄，即葡萄糖和果糖，进行同型发酵。然而，基因组测序和平均核苷酸同一性（ANI）估计显示，与 DSM 23037（迄今为止唯一测序的 菌株）的 ANI 值为 87.44。当 He02 与 DSM 23037 菌株比较时，使用类型菌株基因组服务器工具估计数字 DNA-DNA 杂交值为 30.5%。这些结果表明菌株 He02 构成一个新物种，为此提出名为 sp. nov. 的名称，以 He02（=CECT 31001=DSM 117324=CCM 9395）作为模式菌株。
