Division of Basic Biomedical Sciences, Sanford School of Medicine of the University of South Dakota, Vermillion, SD 57069, USA.
Reprod Sci. 2010 Sep;17(9):809-22. doi: 10.1177/1933719110371854. Epub 2010 Jul 2.
This study tested the hypothesis that reciprocal communication occurs between macrophages and cultured human endometrial stromal cells and that this communication may contribute to the pathology of endometriosis. An endometrial stromal cell line (telomerase-immortalized human endometrial stromal cell [T-HESC]) was treated with macrophage-conditioned medium (CM) +/- estradiol + progesterone. Macrophages were treated without or with T-HESC CM. DNA microarray identified 716 differentially expressed genes in T-HESCs in response to factors secreted by macrophages. Upregulated genes in T-HESC included interleukin 8 (IL-8)/chemokine (C-X-C motif) ligand 8 (CXCL8), matrix metalloproteinase 3 (MMP3), phospholamban, cysteine-rich angiogenic inducer 61 (CYR61), connective tissue growth factor (CTGF), tenascin C, and nicotinamide N-methyltransferase (NNMT), whereas integrin alpha-6 was downregulated. In contrast, 15 named genes were differentially expressed in macrophages in response to factors secreted by endometrial stromal cells. The data document reciprocal communication between macrophages and endometrial stromal cells and suggest that interaction with macrophages stimulates the expression of genes in endometrial stromal cells that may support the establishment of endometriosis.
这项研究检验了这样一个假设，即巨噬细胞和培养的人子宫内膜基质细胞之间存在相互交流，这种交流可能有助于子宫内膜异位症的病理学。使用巨噬细胞条件培养基（CM）+/-雌二醇+孕酮处理子宫内膜基质细胞系（端粒酶永生化人子宫内膜基质细胞[T-HESC]）。未经处理或用 T-HESC CM 处理巨噬细胞。DNA 微阵列鉴定出 716 个在 T-HESC 中对巨噬细胞分泌的因子有反应的差异表达基因。T-HESC 中上调的基因包括白细胞介素 8（IL-8）/趋化因子（C-X-C 基序）配体 8（CXCL8）、基质金属蛋白酶 3（MMP3）、磷酸化肌浆球蛋白结合蛋白、富含半胱氨酸的血管生成诱导因子 61（CYR61）、结缔组织生长因子（CTGF）、腱生蛋白 C 和烟酰胺 N-甲基转移酶（NNMT），而整合素 alpha-6 下调。相比之下，有 15 个命名基因在巨噬细胞中对子宫内膜基质细胞分泌的因子有差异表达。这些数据记录了巨噬细胞和子宫内膜基质细胞之间的相互交流，并表明与巨噬细胞的相互作用刺激了子宫内膜基质细胞中可能支持子宫内膜异位症建立的基因表达。
