Cancer Research UK, 44 Lincoln's Inn Fields, London WC2A 3PX, UK.
Nucleic Acids Res. 2010 Nov;38(20):6968-75. doi: 10.1093/nar/gkq580. Epub 2010 Jul 3.
Removal of the conserved telomere protein, Pot1, confers the immediate loss of fission yeast telomeres. This drastic phenotype has established the centrality of Pot1 for telomere maintenance but prohibited elucidation of the intermediate steps leading to telomere loss. To circumvent this problem, we have generated a conditional allele, pot1-1. We show that loss of Pot1 function during G1 leads to rapid telomere erosion during the ensuing S/G2 period. Precipitous telomere loss depends upon S-phase progression and is preceded by 5' telomeric resection. Telomere loss is accompanied by ATR- and Chk1-mediated checkpoint activation, but is not caused by checkpoint arrest.
去除保守的端粒蛋白 Pot1，会导致裂殖酵母端粒的即刻丢失。这种剧烈的表型确立了 Pot1 在端粒维持中的核心地位，但阻碍了对导致端粒丢失的中间步骤的阐明。为了解决这个问题，我们生成了一个条件性等位基因 pot1-1。我们表明，在 G1 期间失去 Pot1 功能会导致随后的 S/G2 期内端粒迅速侵蚀。急剧的端粒丢失依赖于 S 期的进展，并且在 5'端粒切除之前发生。端粒丢失伴随着 ATR 和 Chk1 介导的检查点激活，但不是由检查点阻滞引起的。
