Han L Q, Li H J, Wang Y Y, Zhu H S, Wang L F, Guo Y J, Lu W F, Wang Y L, Yang G Y
College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Genet Mol Res. 2010 Jun 29;9(2):1250-7. doi: 10.4238/vol9-2gmr814.
The functions of distinct isoforms of solute carrier family 27 transporters (SLC27A1-6), acetyl-CoA carboxylase (ACACA, ACACB), stearoyl-CoA desaturase (SCD1-4), fatty acid desaturase (FADS1-3), LPIN (LPIN1-3), insulin-induced gene (INSIG1, 2), and peroxisome proliferator-activated receptor gamma coactivator1 (PPARGC1A, B) were studied in the mouse mammary gland from pregnancy to lactation. The relative mRNA abundance and percent change in real-time PCR were determined. mRNA expression of SLC27A3 and SLC27A4 was 37- and 1.4-fold more upregulated at 12 days of lactation, respectively (P < 0.01). Transcripts of SCD isoforms were the most abundant, accounting for 59% of all genes measured, and PPARGC1 isoforms were the least (0.06% of all genes measured). The mRNA abundance from ACC, FADS and LPIN accounted for 29, 9 and 2.6%, respectively. INSIG1 mRNA expression was 32-fold more upregulated (P < 0.05), while PPARGC1B was 0.18-fold downregulated at 18 days of lactation (P < 0.01). We concluded that mRNA abundance and expression of these isoforms are affected by the stage of lactation.
在小鼠乳腺从怀孕到哺乳期间，研究了溶质载体家族27转运蛋白（SLC27A1 - 6）、乙酰辅酶A羧化酶（ACACA、ACACB）、硬脂酰辅酶A去饱和酶（SCD1 - 4）、脂肪酸去饱和酶（FADS1 - 3）、LPIN（LPIN1 - 3）、胰岛素诱导基因（INSIG1、2）以及过氧化物酶体增殖物激活受体γ共激活因子1（PPARGC1A、B）不同亚型的功能。测定了实时定量PCR中的相对mRNA丰度和百分比变化。在哺乳第12天，SLC27A3和SLC27A4的mRNA表达分别上调37倍和1.4倍（P < 0.01）。SCD亚型的转录本最为丰富，占所有检测基因的59%，而PPARGC1亚型最少（占所有检测基因的0.06%）。ACC、FADS和LPIN的mRNA丰度分别占29%、9%和2.6%。在哺乳第18天，INSIG1的mRNA表达上调32倍（P < 0.05），而PPARGC1B下调0.18倍（P < 0.01）。我们得出结论，这些亚型的mRNA丰度和表达受哺乳阶段的影响。
