Department of Chemistry and Biochemistry, California State University, Los Angeles, CA, USA.
Electrophoresis. 2010 Aug;31(15):2520-5. doi: 10.1002/elps.201000067.
This paper describes the design, development and application of microfluidic "thin chips" fabricated from PDMS. Thin chips consist of multiple layers of PDMS chemically bonded onto each other. Unlike thicker PDMS chips that suffer from lack of sensitivity due to PDMS absorption in the VIS and UV range, the thinness of these chips allows for the detection of chromophoric species within the microchannel via an external fiber optics detection system. C18-modified reversed-phase silica particles are packed into the microchannel using a temporary taper created by a magnetic valve and separations using both pressure- and electrochromatographic-driven methods are detailed.
本文描述了基于 PDMS 的微流控“薄芯片”的设计、开发和应用。薄芯片由多层 PDMS 通过化学键合而成。与由于 PDMS 在可见和紫外范围内吸收而导致灵敏度不足的较厚 PDMS 芯片不同，这些薄芯片的厚度允许通过外部光纤检测系统检测微通道内的发色团物质。使用磁阀产生的临时锥形将 C18 修饰的反相硅胶颗粒填充到微通道中，并详细介绍了使用压力和电色谱驱动方法进行分离。
