Dipartimento di Scienze del Farmaco, Facoltà di Farmacia-Università degli Studi G. D'Annunzio Chieti-Pescara, Chieti, Italy.
J Sep Sci. 2010 Aug;33(16):2411-6. doi: 10.1002/jssc.201000151.
Only a small proportion of old specimens contain DNA that can be amplified by PCR. Therefore, rapid screening methods are crucial to identify the large fraction of samples that are so badly preserved that there is no need to attempt DNA extraction. In particular, the extent of racemization of some amino acids has proved to be a very useful proxy for DNA preservation. In this study, a rigorous method for the determination of the D/L ratio for aspartic acid and alanine by RP-HPLC with fluorescence detection was developed with the aim to obtain a fast and cheap procedure for both sample preparation and amino acids analysis, without compromising precision and accuracy.
只有一小部分的陈旧标本含有可通过 PCR 扩增的 DNA。因此，快速筛选方法对于鉴定那些保存状况极差以致于无需尝试 DNA 提取的大量样本至关重要。特别是，一些氨基酸的外消旋化程度已被证明是 DNA 保存的一个非常有用的替代指标。在这项研究中，开发了一种使用反相高效液相色谱法（RP-HPLC）和荧光检测法测定天冬氨酸和丙氨酸的 D/L 比的严格方法，目的是在不影响精密度和准确性的前提下，获得一种快速且廉价的样品制备和氨基酸分析方法。
