一种根管治疗药物对粪肠球菌生物膜抗菌活性的体外研究。
An in vitro study of the antimicrobial activity of some endodontic medicaments against Enteroccus faecalis biofilms.
出版信息
Aust Dent J. 2010 Jun;55(2):150-5. doi: 10.1111/j.1834-7819.2010.01222.x.
BACKGROUND
The in vitro antimicrobial activity of a series of endodontic medicaments and their bases against biofilms of Enterococcus faecalis was investigated.
METHODS
The medicaments tested were Pulpdent paste, Ledermix paste, a 50:50 Ledermix and Pulpdent mixture, and a replica of Ledermix paste. Bases included methyl cellulose with water, polyethylene glycol (PEG), and PEG with zinc oxide, calcium chloride and the other components (inactives) that make up the Ledermix paste base. Biofilms grown on cellulose nitrate membrane filters were exposed to the medicaments for up to 5 days. The number of surviving colony forming units (CFU) was determined at days 1, 3 and 5. The results were expressed as a bacterial survival index (BSI) when compared to the unexposed control.
RESULTS
Pulpdent produced the greatest reduction of BSI, followed by the 50:50 mixture of Pulpdent and Ledermix pastes. Ledermix paste, its replica and the individual bases showed no significant reductions in the BSI for E. faecalis.
CONCLUSIONS
Within the limitations of this laboratory study, calcium hydroxide containing preparations had greater potential for reducing the survival of E. faecalis in a biofilm environment.
背景
本研究旨在检测一系列根管治疗药物及其基质对粪肠球菌生物膜的体外抗菌活性。
方法
实验中所使用的药物分别为 Pulpdent 糊剂、Ledermix 糊剂、50:50 的 Ledermix 糊剂和 Pulpdent 混合物，以及 Ledermix 糊剂的复制品。所使用的基质包括含有水的甲基纤维素、聚乙二醇（PEG）、以及含有氧化锌、氯化钙和构成 Ledermix 糊剂基质的其他成分（惰性成分）的 PEG。将生物膜种植在硝酸纤维素膜滤器上，然后将其暴露于药物中长达 5 天。在第 1、3 和 5 天，通过平板计数法检测存活的菌落形成单位（CFU）的数量，并计算细菌存活指数（BSI）。与未经处理的对照组相比，BSI 用于表示药物的抗菌效果。
结果
Pulpdent 糊剂产生的 BSI 降低幅度最大，其次是 50:50 的 Pulpdent 和 Ledermix 糊剂混合物。Ledermix 糊剂、其复制品以及各个基质对粪肠球菌生物膜的 BSI 没有显著降低。
结论
在本实验室研究的限制范围内，含氢氧化钙的制剂在生物膜环境中更有可能降低粪肠球菌的存活。
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