Sri Ramaswamy Memorial Dental College, Sri Ramaswamy Memorial University, Chennai, Tamilnadu, India.
J Endod. 2011 Sep;37(9):1287-9. doi: 10.1016/j.joen.2011.05.028. Epub 2011 Jul 16.
The aim of this study was to evaluate and compare the antimicrobial activity of calcium hydroxide, triantibiotic mixture (TAM), and an ethanol extract of propolis as intracanal medicaments on Enterococcus faecalis-infected root canals.
One hundred twenty extracted intact human permanent incisors were decoronated, and chemomechanical preparation of the root canal was performed. After sterilization of the samples, they were inoculated with pure culture of E. faecalis and incubated. After incubation, colony-forming units were recorded before medication. Then, samples were divided randomly into five groups (n = 24). Each group was then exposed to various intracanal medicaments, namely calcium hydroxide (group 1), TAM (group 2), propolis (group 3), ethanol (group 4), and saline as the control group (group V). The antibacterial effectiveness of the different intracanal medicament was recorded by determining the percentage reduction in colony counts (%RCC) at the end of days 1, 2, and 7. The data were statistically analyzed using one-way analysis of variance and Tukey Honestly Significant Difference (HSD) post hoc test.
The %RCC was highest for propolis showing 100% reduction on day 2 followed by TAM showing 82.5%, 92.2%, and 98.4% of reduction on days 1, 2, and 3, respectively. Calcium hydroxide showed a gradual increase in antibacterial activity with a maximum of 59.4% on day 7.
Propolis was more effective than TAM against E. faecalis at a 2-day time period, and both were equally effective at 7 days.
本研究旨在评估和比较氢氧化钙、三抗生素混合物（TAM）和蜂胶乙醇提取物作为根管内药物对粪肠球菌感染根管的抗菌活性。
将 120 颗完整的人恒切牙进行去冠处理，并进行根管的化学机械预备。样本消毒后，用纯粪肠球菌培养物接种并孵育。孵育后，在用药前记录菌落形成单位。然后，将样本随机分为五组（n = 24）。每组分别暴露于不同的根管内药物，即氢氧化钙（第 1 组）、TAM（第 2 组）、蜂胶（第 3 组）、乙醇（第 4 组）和生理盐水作为对照组（第 5 组）。通过确定菌落计数减少百分比（%RCC），在第 1、2 和 7 天记录不同根管内药物的抗菌效果。使用单因素方差分析和 Tukey Honestly Significant Difference (HSD) 事后检验对数据进行统计分析。
第 2 天，蜂胶的 %RCC 最高，达到 100%的减少，其次是 TAM，分别在第 1、2 和 3 天减少 82.5%、92.2%和 98.4%。氢氧化钙的抗菌活性逐渐增加，第 7 天达到最大的 59.4%。
在 2 天的时间内，蜂胶比 TAM 对粪肠球菌更有效，而在 7 天的时间内，两者的效果相当。
