Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, 06097 Halle, Germany.
Histochem Cell Biol. 2010 Aug;134(2):115-27. doi: 10.1007/s00418-010-0721-y. Epub 2010 Jul 7.
The present study dealt with the localization of different calcium-binding proteins (CaBPs) in the pancreatic tissue of non-diabetic and diabetic rats and in rat insulinoma beta-cells (INS-1). Transcripts of CaBPs displayed different expression levels in rat pancreatic tissue and INS-1 cells. Immunohistochemistry demonstrated that three of these proteins, calmodulin, calreticulin and calbindin-D28k, were located predominantly in the pancreatic islets (in both alpha- and beta-cells) of rats, showing weaker labeling of exocrine tissue. Secretagogin was exclusively found within islets. All CaBPs were also immunohistochemically detected in INS-1 cells. Immunohistochemical analysis demonstrates differences in CaBP distributions when comparing the pancreatic tissues of diabetic Goto-Kakizaki rats and non-diabetic Wistar rats. Pancreatic tissue in type 2 diabetic Goto-Kakizaki rats showed significantly higher transcript levels of all CaBPs compared to those in Wistar rats. These results indicate that alterations of CaBPs in pancreatic islets are associated with metabolic disturbances related to type 2 diabetes.
本研究探讨了不同钙结合蛋白（CaBPs）在非糖尿病和糖尿病大鼠胰腺组织以及大鼠胰岛素瘤β细胞（INS-1）中的定位。CaBPs 的转录本在大鼠胰腺组织和 INS-1 细胞中显示出不同的表达水平。免疫组织化学显示，这三种蛋白（钙调蛋白、钙网蛋白和钙结合蛋白-D28k）主要位于大鼠胰腺胰岛（α 和 β 细胞）中，外分泌组织的标记较弱。分泌素仅在胰岛内发现。所有 CaBPs 在 INS-1 细胞中也通过免疫组织化学检测到。免疫组织化学分析表明，比较 2 型糖尿病 Goto-Kakizaki 大鼠和非糖尿病 Wistar 大鼠的胰腺组织时，CaBP 分布存在差异。与 Wistar 大鼠相比，2 型糖尿病 Goto-Kakizaki 大鼠的胰腺组织中所有 CaBPs 的转录本水平显著升高。这些结果表明，胰岛中 CaBPs 的改变与 2 型糖尿病相关的代谢紊乱有关。
