Ladefoged S A, Christiansen G
Institute of Medical Microbiology, University of Aarhus, Denmark.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):133-9. doi: 10.1016/0378-1097(91)90075-l.
A gene from Mycoplasma hominis PG21 similar to the tuf gene encoding the elongation factor Tu (EF-Tu) of Escherichia coli was cloned and sequenced. The 1193-bp open reading frame flanked by a putative promoter and a potential stem-and-loop structure encoded a 44-kDa polypeptide. The tuf gene of M. hominis PG21 has the lowest G + C content seen in prokaryotes (38.2%). A gene (mhlmp1) encoding a variable surface exposed membrane protein (LMP1) was found downstream the 3' end of the tuf gene. It was found that the highly conserved tuf gene was linked to the highly variable mhlmp1 gene in 26 different M. hominis strains.
克隆并测序了来自人型支原体PG21的一个基因，该基因与编码大肠杆菌延伸因子Tu（EF-Tu）的tuf基因相似。由一个推定的启动子和一个潜在的茎环结构侧翼的1193 bp开放阅读框编码一个44 kDa的多肽。人型支原体PG21的tuf基因具有原核生物中所见的最低G + C含量（38.2%）。在tuf基因3'端下游发现了一个编码可变表面暴露膜蛋白（LMP1）的基因（mhlmp1）。发现在26种不同的人型支原体菌株中，高度保守的tuf基因与高度可变的mhlmp1基因相连。
