人型支原体编码赖氨酰 - tRNA合成酶（LysRS）基因的特性分析。

Characterization of a Mycoplasma hominis gene encoding lysyl-tRNA synthetase (LysRS).

作者信息

Ozkökmen D, Birkelund S, Christiansen G

机构信息

Institute of Medical Microbiology, University of Aarhus, Denmark.

出版信息

FEMS Microbiol Lett. 1994 Mar 1;116(3):277-82. doi: 10.1111/j.1574-6968.1994.tb06715.x.

DOI:10.1111/j.1574-6968.1994.tb06715.x

PMID:8181699

Abstract

The gene encoding lysyl-tRNA synthetase (lysS) in Mycoplasma hominis was cloned and sequenced. The gene was found to have an open reading frame of 1466 bp encoding a polypeptide with a predicted molecular mass of 57 kDa. The amino acid sequence showed 44.3% and 43.7% identity to the Escherichia coli lysyl-tRNA synthetases, encoded by lysS and lysU. Only one lysyl-tRNA synthetase encoding gene was found in M. hominis. The G + C content of the gene was found to be 28.6%, which is significantly lower than in other prokaryotes. The gene was located 4 kb upstream of the M. hominis PG21 rRNA B operon.

摘要

人型支原体中编码赖氨酰 - tRNA合成酶（lysS）的基因被克隆并测序。该基因有一个1466 bp的开放阅读框，编码一个预测分子量为57 kDa的多肽。氨基酸序列与大肠杆菌中由lysS和lysU编码的赖氨酰 - tRNA合成酶分别有44.3%和43.7%的同一性。人型支原体中仅发现一个编码赖氨酰 - tRNA合成酶的基因。该基因的G + C含量为28.6%，明显低于其他原核生物。该基因位于人型支原体PG21 rRNA B操纵子上游4 kb处。