Car N B, Woods D R
Department of Medicine, University of the Witwatersrand Medical School, Parktown, Africa.
FEMS Microbiol Lett. 1991 Apr 15;63(2-3):297-301. doi: 10.1016/0378-1097(91)90102-g.
Vibrio strain 14 supports phage alpha 3a growth in standing stationary phase cells but not in shaking (aerated) stationary phase cells. In exponential cells, protein was turned over at 1.8% h-1, and the rate was increased by starvation or inhibition of protein synthesis. In shaking stationary phase cells the rate of protein turnover was low (1.0% h-1) for proteins synthesised during growth but high (20% h-1) for recently synthesised proteins. In contrast recently synthesised proteins in standing stationary phase cells were stable over 60 min and proteins synthesised during growth were turned over at 2.9% h-1. ppGpp and pppGpp were detected in exponential cells, but were not detected in stationary phase cells.
弧菌菌株14支持噬菌体α 3a在静置静止期细胞中生长，但不支持在振荡（通气）静止期细胞中生长。在指数生长期细胞中，蛋白质的更新率为1.8%/小时，饥饿或蛋白质合成抑制会提高该速率。在振荡静止期细胞中，生长期间合成的蛋白质的更新率较低（1.0%/小时），但最近合成的蛋白质的更新率较高（20%/小时）。相比之下，静置静止期细胞中最近合成的蛋白质在60分钟内是稳定的，生长期间合成的蛋白质的更新率为2.9%/小时。在指数生长期细胞中检测到了ppGpp和pppGpp，但在静止期细胞中未检测到。
