指数生长期和稳定期弧菌细胞中的蛋白质周转

Protein turnover in exponential and stationary phase Vibrio cells.

作者信息

Car N B, Woods D R

机构信息

Department of Medicine, University of the Witwatersrand Medical School, Parktown, Africa.

出版信息

FEMS Microbiol Lett. 1991 Apr 15;63(2-3):297-301. doi: 10.1016/0378-1097(91)90102-g.

DOI:10.1016/0378-1097(91)90102-g

PMID:2060764

Abstract

Vibrio strain 14 supports phage alpha 3a growth in standing stationary phase cells but not in shaking (aerated) stationary phase cells. In exponential cells, protein was turned over at 1.8% h-1, and the rate was increased by starvation or inhibition of protein synthesis. In shaking stationary phase cells the rate of protein turnover was low (1.0% h-1) for proteins synthesised during growth but high (20% h-1) for recently synthesised proteins. In contrast recently synthesised proteins in standing stationary phase cells were stable over 60 min and proteins synthesised during growth were turned over at 2.9% h-1. ppGpp and pppGpp were detected in exponential cells, but were not detected in stationary phase cells.

摘要

弧菌菌株14支持噬菌体α 3a在静置静止期细胞中生长，但不支持在振荡（通气）静止期细胞中生长。在指数生长期细胞中，蛋白质的更新率为1.8%/小时，饥饿或蛋白质合成抑制会提高该速率。在振荡静止期细胞中，生长期间合成的蛋白质的更新率较低（1.0%/小时），但最近合成的蛋白质的更新率较高（20%/小时）。相比之下，静置静止期细胞中最近合成的蛋白质在60分钟内是稳定的，生长期间合成的蛋白质的更新率为2.9%/小时。在指数生长期细胞中检测到了ppGpp和pppGpp，但在静止期细胞中未检测到。