Quigley J D, Heitmann R N
Dept. of Anim. Sci., University of Tennessee, Knoxville 37901.
J Anim Sci. 1991 Mar;69(3):1178-87. doi: 10.2527/1991.6931178x.
Four nonlactating, nonpregnant, mature ewes equipped with multiple venous and arterial catheters were used to evaluate the influence of propionate as a satiety signal in ruminants. Our experiment was a 4 x 4 Latin square with portal infusion (physiological saline [Sa] or sodium propionate [Pr]) and DE intake (Lo, 63% of maintenance requirement, or Hi, 200% of maintenance requirement) as factors. One 240-min infusion of Pr (1 mmol/min) or Sa into the portal vein began at approximately 0800 on d 8 of each 8-d period. Feed intake was measured and hepatic blood was sampled every 30 min during infusion. Intake of DM and digestible energy (DEI) during infusion were not affected by infusion or diet and were most rapid at 30 min postfeeding. Average 30-min DMI and DEI were 539 g and 1,484 kcal, respectively, at 240 min. Cumulative DMI and DEI were unaffected by infusion but tended to be greater with Lo. After 30 min, animals tended to consume Lo at a greater rate than Hi, suggesting that satiety was delayed. Insulin concentration was increased (P less than .02) when animals consumed Hi (36.1 mU/liter) vs Lo (16.8 mU/liter) and was elevated (P less than .01) at 30 and 60 min postfeeding when animals were infused with Pr. Plasma acetate tended to be reduced with Pr infusion. Plasma Pr tended to increase with Pr infusion, especially when sheep were fed Lo. Satiety, DMI, and DEI were not affected by Pr infusion in this study.
选用4只非泌乳、非妊娠的成年母羊，通过在其体内植入多根动静脉导管，来评估丙酸盐作为反刍动物饱腹感信号的影响。本实验采用4×4拉丁方设计，以门静脉灌注（生理盐水[Sa]或丙酸钠[Pr]）和可消化能量摄入量（低，维持需要量的63%；或高，维持需要量的200%）作为实验因素。在每个8天周期的第8天，大约08:00开始向门静脉内灌注1次Pr（1 mmol/min）或Sa，持续240分钟。灌注期间每30分钟测量1次采食量，并采集肝血样。灌注期间的干物质采食量（DMI）和可消化能量摄入量（DEI）不受灌注或日粮的影响，且在采食后30分钟时最高。在240分钟时，平均30分钟的DMI和DEI分别为539克和1484千卡。累积DMI和DEI不受灌注影响，但低能量组的数值往往更高。30分钟后，动物采食低能量日粮的速度往往高于高能量日粮，这表明饱腹感延迟。当动物采食高能量日粮（36.1 mU/升）与低能量日粮（16.8 mU/升）时，胰岛素浓度升高（P<0.02），并且在动物灌注Pr后的采食后30分钟和60分钟时胰岛素浓度升高（P<0.01）。灌注Pr后，血浆乙酸盐浓度有降低趋势。灌注Pr后，血浆丙酸盐浓度有升高趋势，尤其是在给绵羊饲喂低能量日粮时。在本研究中，饱腹感、DMI和DEI不受Pr灌注的影响。
