CD74 是结肠上皮细胞的存活受体。
CD74 is a survival receptor on colon epithelial cells.
机构信息
Department of Immunology, Weizmann Institute of Science, Rehovot 76100, Israel.
出版信息
World J Gastroenterol. 2010 Jul 14;16(26):3258-66. doi: 10.3748/wjg.v16.i26.3258.
Abstract
AIM
To investigate the expression and function of CD74 in normal murine colon epithelial cells (CEC) and colon carcinoma cells.
METHODS
Expression of CD74 mRNA and protein were measured by reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting and fluorescence-activated cell sorter (FACS). The effect of migration inhibitory factor (MIF) on the survival of normal CEC from C57BL/6, NOD/SCID, and CD74 deficient mice both in vitro and in vivo, and on the CT26 carcinoma cell line was analyzed by (quantitative) qRT-PCR, RT-PCR, Western blotting and FACS.
RESULTS
CD74 was found to be expressed on normal CEC. Stimulation of CD74 by MIF induced a signaling cascade leading to up-regulation of Bcl-2 expression, resulting in a significant increased survival of CEC. CD74 was also expressed on the CT26 colon carcinoma cell line and its stimulation by MIF resulted in enhanced cell survival, up-regulation of Akt phosphorylation and Bcl-2 expression.
CONCLUSION
CD74 is expressed on CEC and colon carcinoma cells and serves as a survival receptor in these cells. These results may have implications on colorectal cancer research.
摘要
目的
研究 CD74 在正常鼠结肠上皮细胞（CEC）和结肠癌细胞中的表达和功能。
方法
采用逆转录-聚合酶链反应（RT-PCR）、Western blot 和流式细胞术（FACS）检测 CD74 mRNA 和蛋白的表达。分析迁移抑制因子（MIF）对 C57BL/6、NOD/SCID 和 CD74 缺陷型小鼠正常 CEC 体外和体内生存能力的影响，以及对 CT26 癌细胞系的影响，采用（定量）qRT-PCR、RT-PCR、Western blot 和 FACS 进行分析。
结果
发现 CD74 在正常 CEC 上表达。MIF 刺激 CD74 诱导信号级联反应，导致 Bcl-2 表达上调，从而显著增加 CEC 的存活。CD74 也在 CT26 结肠癌细胞系上表达，MIF 刺激其导致细胞存活增强，Akt 磷酸化和 Bcl-2 表达上调。
结论
CD74 在 CEC 和结肠癌细胞上表达，并作为这些细胞中的存活受体。这些结果可能对结直肠癌研究具有重要意义。
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