Department of Chemistry, University of California, Riverside, California 92521, USA.
J Am Chem Soc. 2010 Aug 4;132(30):10383-90. doi: 10.1021/ja102252d.
This paper details the incorporation of a water-soluble deep cavitand into a membrane bilayer assembled onto a nanoglassified surface for study of molecular recognition in a membrane-mimicking setting. The cavitand retains its host properties, and real-time analysis of the host:guest properties of the membrane:cavitand complex via surface plasmon resonance and fluorescence microscopy is described. The host shows selectivity for choline-derived substrates, and no competitive incorporation of substrate is observed in the membrane bilayer. A variety of trimethylammonium-derived substrates are suitable guests, displaying varied binding affinities in a millimolar range. The membrane:cavitand:guest complexes can be subsequently used to capture NeutrAvidin protein at the membrane surface if a biotin-derived guest molecule is used. The surface coverage of NeutrAvidin is affected by the spacer used to derivatize the biotin. Increased distance from the bilayer allows a higher concentration of protein to be immobilized, suggesting a diminishing detrimental steric effect when the binding event is shifted away from the surface.
本文详细介绍了将一种水溶性深穴状化合物纳入组装在纳米分类表面上的膜双层中，以在模拟膜的环境中研究分子识别。穴状化合物保留了其主体性质，并通过表面等离子体共振和荧光显微镜实时分析了膜-穴状化合物复合物的主体-客体性质。主体对胆碱衍生的底物表现出选择性，并且在膜双层中没有观察到底物的竞争性纳入。各种三甲铵衍生的底物是合适的客体，在毫摩尔范围内显示出不同的结合亲和力。如果使用生物素衍生的客体分子，则可以将膜:穴状化合物:客体复合物随后用于在膜表面捕获 NeutrAvidin 蛋白。用于衍生生物素的间隔物会影响 NeutrAvidin 的表面覆盖率。与双层的距离增加允许固定更高浓度的蛋白质，这表明当结合事件从表面转移时，结合事件的有害空间位阻效应会减小。
