Defence Laboratory, Defence Research & Development Organization, Jodhpur 342011, India.
J Tissue Viability. 2011 May;20(2):49-54. doi: 10.1016/j.jtv.2010.06.001. Epub 2010 Jul 8.
Dried amniotic membrane contains collagen matrix and key bioactive molecules like fibronectin, laminin, glycosaminoglycans and elastin. Fresh and cryopreserved human amniotic membrane has been widely explored as a biological dressing. However, fresh and cryopreserved amniotic membranes are not readily available or require special storage conditions. This investigation was aimed to study the functional and clinical efficacy of air-dried radiation sterilized amniotic membranes as dressing in burn wound care.
Amniotic membranes collected from placentae of screened donors were processed and sterilized by gamma radiation at 25 kGy. The fluid handling capacity, shelf life and clinical efficacy of air-dried gamma-irradiated amniotic membranes was evaluated.
Fluid handling capacity of the air-dried irradiated amniotic membrane dressing was 3.79-4.2 g/10 cm(2) in 24 h. Infrared (IR) spectral scanning showed no degradation or change in the dried gamma-irradiated amniotic tissue after 2 and 5 years of storage. No effect of storage on the impermeability of the processed amniotic membranes to bacteria was observed. The dried gamma-irradiated amniotic membranes even after 5 years of storage provided an effective barrier to microbial penetration. The dried amniotic membranes were applied to 22 cases of scald, flame and electrical burns. Of the 22 patients studied, 19 patients had excellent results in the form of complete epithelialization of wound with an average healing time of 15-25 days.
IR studies and microbial permeability test indicate no qualitative changes in the material property of dried gamma-irradiated amniotic membranes after 2 and 5 years of storage. Air-dried amniotic membrane with the advantage of storage at room temperature as well as functional efficiency is an ideal dressing for burn wound care.
干燥羊膜含有胶原基质和关键的生物活性分子，如纤维连接蛋白、层粘连蛋白、糖胺聚糖和弹性蛋白。新鲜和冷冻保存的人羊膜已被广泛探索作为生物敷料。然而，新鲜和冷冻保存的羊膜不易获得或需要特殊的储存条件。本研究旨在研究干燥辐照消毒羊膜作为烧伤创面敷料的功能和临床疗效。
从筛选供体的胎盘收集羊膜，并在 25 kGy 下进行伽马辐射处理和消毒。评估干燥辐照羊膜的流体处理能力、货架寿命和临床疗效。
干燥辐照羊膜敷料在 24 小时内的流体处理能力为 3.79-4.2 g/10 cm(2)。红外（IR）光谱扫描显示，经过 2 年和 5 年储存后，干燥辐照羊膜组织没有降解或变化。储存对处理后的羊膜的细菌渗透性没有影响。即使经过 5 年储存，干燥辐照羊膜仍能有效防止微生物渗透。将干燥辐照羊膜应用于 22 例烫伤、火焰和电烧伤患者。在研究的 22 例患者中，19 例患者的伤口完全上皮化，愈合时间平均为 15-25 天，效果极佳。
IR 研究和微生物渗透性测试表明，干燥辐照羊膜在经过 2 年和 5 年后，其材料性质没有发生质的变化。干燥的羊膜具有在室温下储存的优势，且功能效率高，是一种理想的烧伤创面敷料。
