胎盘滋养层细胞中端粒在宫内生长受限（IUGR）的妊娠中较短。
Telomeres are shorter in placental trophoblasts of pregnancies complicated with intrauterine growth restriction (IUGR).
机构信息
Department of OBGYN, Meir Medical Center, Kfar Saba, Israel.
出版信息
Early Hum Dev. 2010 Jul;86(7):451-6. doi: 10.1016/j.earlhumdev.2010.06.002.
OBJECTIVE
Telomeres are nucleoprotein structures located at the termini of chromosomes, and protect them from fusion and degradation. Telomeres are progressively shortened with each mitotic cycle and by environmental factors. We hypothesized that antepartum stress can lead to accelerated telomere shortening in placental trophoblasts, and plays a role in intrauterine growth restriction (IUGR).
METHODS
Placental biopsies were derived from 16 pregnancies complicated with IUGR and from 13 uncomplicated pregnancies. Fluorescence-in-situ protocol was used to determine telomere length. Immunohistochemistry for hTERT was performed to assess telomerase activity. Clinical and histopathological characteristics were collected to ensure that IUGR was secondary to placental insufficiency. Fluorescence-in-situ-hybridization was used to rule out aneuploidy as a reason for shortened telomeres.
RESULTS
The number and intensity of telomeres staining and telomerase activity were significantly lower in the IUGR placentas. No aneuploidy was detected for the chromosomes checked in the placental biopsies.
CONCLUSIONS
Telomeres are shorter in trophoblasts of IUGR placentas.
目的
端粒是位于染色体末端的核蛋白结构，可防止染色体融合和降解。端粒在每次有丝分裂循环和环境因素的作用下逐渐缩短。我们假设产前应激可导致胎盘滋养细胞中端粒缩短加速，并在宫内生长受限（IUGR）中发挥作用。
方法
从 16 例 IUGR 合并妊娠和 13 例无并发症妊娠中获取胎盘活检。使用荧光原位杂交方案来确定端粒长度。进行 hTERT 的免疫组织化学染色以评估端粒酶活性。收集临床和组织病理学特征以确保 IUGR 是继发于胎盘功能不全。荧光原位杂交排除了非整倍体作为端粒缩短的原因。
结果
IUGR 胎盘中端粒的数量和强度染色以及端粒酶活性明显降低。在胎盘活检中检查的染色体未发现非整倍体。
结论
IUGR 胎盘滋养细胞中端粒较短。
Zotero 插件