Key Laboratory of Microbiological Engineering of Agricultural Environment, Nanjing Agricultural University, Nanjing, China.
Plasmid. 2010 Nov;64(3):200-3. doi: 10.1016/j.plasmid.2010.06.006. Epub 2010 Jul 8.
The complete nucleotide sequence of a cryptic plasmid pPZZ84 from Bacillus pumilus strain ZZ84 was determined. Plasmid pPZZ84 is 6817bp long with GC content of 36.7%. Seven putative open reading frames were identified. ORF7 shows 91% and 90% amino acid identity with rep proteins of pSH1452 and pPL1, respectively, members of rolling-circle replication (RCR) pC194-family. A typical pC194-family double strand origin (dso), a single-stranded origin (sso) and rap (regulator aspartate phosphatase) proteins were also identified in the plasmid. These results imply that pPZZ84 belongs to the Bacillus subtilis species group of small rolling circle (BsSRC) replicating plasmids. The plasmid copy number of pPZZ84 in B. pumilus ZZ84 was estimated to be 46 per cell, more than that of other BsSRC plasmids in their hosts.
从芽孢杆菌菌株 ZZ84 中分离出一个隐秘质粒 pPZZ84，其完整的核苷酸序列已被确定。质粒 pPZZ84 长 6817bp，GC 含量为 36.7%。鉴定出了 7 个可能的开放阅读框。ORF7 与 pSH1452 和 pPL1 的 rep 蛋白分别具有 91%和 90%的氨基酸同一性，这两个蛋白都是属于滚环复制（RCR）pC194 家族的成员。该质粒中还鉴定出了一个典型的 pC194 家族双链起始点（dso）、一个单链起始点（sso）和 rap（天冬氨酸磷酸酶调节蛋白）蛋白。这些结果表明，pPZZ84 属于枯草芽孢杆菌小滚环（BsSRC）复制质粒的物种群。在芽孢杆菌 ZZ84 中，pPZZ84 的质粒拷贝数估计为每个细胞 46 个，高于其宿主中其他 BsSRC 质粒的拷贝数。
