Shen Y, Wang Y, Li D, Wang C, Xu B, Dong G, Huang H, Jing H
Department of Cardio-thoracic Surgery, Jinling Hospital, Clinical Medicine School, Nanjing University, Nanjing, China.
Transplant Proc. 2010 Jun;42(5):1595-7. doi: 10.1016/j.transproceed.2009.11.050.
Ischemia-reperfusion (I/R) injury may influence graft function after transplantation. Erythropoietin (EPO) attenuates I/R injury in various animal organs such as intestine, brain, and kidney.
To evaluate the effects of pretreatment with recombinant human EPO (rhEPO) on I/R-induced heart injury.
A rat model of I/R injury was established by ligating the left descending coronary artery for 30 minutes, followed by reperfusion for 4 hours. Fifty Sprague-Dawley rats were divided into 5 groups: sham operation; I/R; I/R+rhEPO, 100 U/kg; I/R+rhEPO, 1000 U/kg; and I/R+rhEPO, 5000 U/kg. Electrocardiograms were assessed continuously to note arrhythmia caused by reperfusion. Serum concentrations of interleukin (IL)-6 and IL-8, and tumor necrosis factor-alpha were measured at 2 and 4 hours after reperfusion.
The rhEPO-treated animals exhibited dosage-dependent significant reduction in the incidence of ventricular arrhythmia caused by reperfusion, and markedly decreased serum concentrations of IL-6, IL-8, and tumor necrosis factor-alpha (P < .05) compared with the I/R group (P < .05).
The rhEPO attenuates myocardial I/R injury in rats, at least in part related to inhibition of the system inflammatory response.
