Hackl F, Stiegler P, Stadlbauer V, Schaffellner S, Iberer F, Matzi V, Maier A, Klemen H, Smolle-Jüttner F M, Tscheliessnigg K
Department of Surgery, Division of Transplantation Surgery Medical University of Gray, Austria.
Transplant Proc. 2010 Jun;42(5):1621-3. doi: 10.1016/j.transproceed.2010.02.071.
Organ preservation quality impacts porcine islet cell isolation and transplantation success. Among several preservation methods, the two-layer method is promising, but technically demanding and fails to deliver sufficient oxygen. The use of hyperbaric oxygenation may be an easier, more effective method to supply high partial pressure of oxygen (pO(2)) for organ storage. Therefore, the aim of this study was to test the capability of preoxygenation of various preservation solutions with HBO to maintain high pO(2) levels.
University of Wisconsin (UW), Custodiol, Perfadex, or Celsior solutions were preoxygenated in a pressure chamber. NaCl served as the control. pO(2) levels were measured at defined times. The oxygen storage capability was evaluated by leaving the storage bottles open for 2 minutes.
It was feasible to preoxygenate preservation solutions. The best solution to maintain high pO(2) tensions was Perfadex, followed by Celsior, and UW.
The greater the amount of oxygen in the preservation solution, the more oxygen can be delivered to the preserved pancreas. Further studies on the influence of preoxygenated preservation solutions on the porcine pancreas are warranted to improve organ quality, porcine islet cell isolation, and transplantation success.
器官保存质量会影响猪胰岛细胞的分离和移植成功率。在多种保存方法中，双层法很有前景，但技术要求高且无法提供充足的氧气。使用高压氧合可能是一种更简便、更有效的方法，可为器官储存提供高氧分压（pO₂）。因此，本研究的目的是测试用高压氧对各种保存溶液进行预氧合以维持高pO₂水平的能力。
将威斯康星大学（UW）溶液、Custodiol溶液、Perfadex溶液或Celsior溶液在压力舱中进行预氧合。氯化钠用作对照。在规定时间测量pO₂水平。通过将储存瓶打开2分钟来评估氧气储存能力。
对保存溶液进行预氧合是可行的。维持高pO₂张力的最佳溶液是Perfadex溶液，其次是Celsior溶液和UW溶液。
保存溶液中的氧气量越多，能输送到保存的胰腺的氧气就越多。有必要进一步研究预氧合保存溶液对猪胰腺的影响，以提高器官质量、猪胰岛细胞分离和移植成功率。
