Han B, Meng B, Cui G, Wu Z, Yu L, Zhu H, Ma H, Shi J, Lv Y
Department of Hepatobiliary Surgery, the First Affiliated Hospital of Medical College, Xi'an Jiaotong University, Shaanxi, PR China.
Transplant Proc. 2010 Jun;42(5):1944-8. doi: 10.1016/j.transproceed.2010.01.073.
The regeneration of splenic autotransplants remains unsatisfactory. The liver has a rich blood supply and is prone to form collateral vessels to nearby ischemic tissues. It should be valuable to study whether it is a new site to facilitate splenic regeneration.
One hundred twenty Sprague-Dawley rats were randomly divided into 12 groups. After splenectomy, each weighed spleen was attached to the diaphragmatic side of the left hepatic lobe using an adhesive. The rats were sacrificed on postoperative day(POD)s 1, 3, 7, 14, 21, 28, 35, 42, 49, 56, 70, and 84. Six spleens in each group were dissected for weighing and another 4 for histologic analysis (H&E). Splenic autografts of another 12 rats attached to the mesenterium were sacrificed on POD 28, 56, and 84 for histologic analysis (H&E).
The spleens attached to the liver underwent almost complete necrosis and then regenerated centripetally into structures comparable to a normal spleen, with typical red and white pulps as well as a marginal zone on POD 35. The mass of the implants recovered to more than two fifths of the original (44.2% +/- 3.6%) on POD 84. For splenic autografts attached to mesenterium, typical red and white pulps were observed on POD 56. The marginal zone was not clear on POD 84.
The liver proved to be an appropriate site for splenic autotransplantation. Regeneration was histologically superior to that of splenic tissue attached to the mesenterium. The application of a tissue adhesive may be a new technique for splenic autotransplantation.
脾自体移植的再生情况仍不尽人意。肝脏血供丰富，易于形成侧支血管至附近缺血组织。研究肝脏是否为促进脾再生的新部位应具有重要价值。
120只Sprague-Dawley大鼠随机分为12组。脾切除术后，将每个称质量的脾脏用粘合剂附着于左肝叶的膈面。分别于术后第1、3、7、14、21、28、35、42、49、56、70和84天处死大鼠。每组取6个脾脏称重，另取4个进行组织学分析（苏木精-伊红染色）。另外12只附着于肠系膜的脾自体移植大鼠分别于术后第28、56和84天处死进行组织学分析（苏木精-伊红染色）。
附着于肝脏的脾脏几乎完全坏死，随后向心性再生为与正常脾脏相当的结构，在术后第35天可见典型的红髓和白髓以及边缘区。术后第84天，植入脾脏的质量恢复至超过原来的五分之二（44.2%±3.6%）。对于附着于肠系膜的脾自体移植，术后第56天可见典型的红髓和白髓。术后第84天边缘区不清晰。
肝脏被证明是脾自体移植的合适部位。组织学上其再生优于附着于肠系膜的脾组织。组织粘合剂的应用可能是一种新的脾自体移植技术。
