Boston University School of Medicine, Boston, Massachusetts 02118, USA.
Laryngoscope. 2010 Sep;120(9):1744-8. doi: 10.1002/lary.21068.
To determine if monocytes activated toward an angiogenic phenotype can be used to improve ischemic tissue healing in a rat skin flap model.
Prospective experimental study on Wistar rats.
A caudally based 9 x 3 cm dorsal skin/panniculus carnosus flap was raised in 15 rats. The animals were divided into three groups: the monocyte group (N = 5) received subcutaneous topical application of 0.1-0.2 cc of i-Monogrid, a collagen gel containing M2 angiogenic monocytes; control group 1 (N = 5) received application of cell-free collagen; and control group 2 (N = 5) received no treatment. Skin flaps were stapled in place and observed for wound ischemia and necrosis of the skin flap. One week postoperatively, skin and underlying muscle were harvested for histologic analyses.
No macroscopic differences in wound healing or microscopic differences in skin viability were observed. However, the monocyte group showed significantly greater vascular improvement than C1 (P = .047, chi = 3.96), and a trend toward greater vascular improvement than C2 (P = .103, chi = 2.67).
Delivery of activated pro-angiogenic monocytes to an ischemic skin flap tended to improve histologic evidence of vascularity without corresponding microscopic or gross evidence of improved flap survival. These results are encouraging regarding the use of monocytes as a potential method of improving vascularization of ischemic tissue.
确定是否可以将向血管生成表型激活的单核细胞用于改善大鼠皮瓣模型中的缺血组织愈合。
对 Wistar 大鼠进行的前瞻性实验研究。
在 15 只大鼠中掀起了一个尾侧为基础的 9 x 3 cm 背侧皮肤/肉膜脂肪瓣。将动物分为三组：单核细胞组（N = 5）接受皮下局部应用 0.1-0.2 cc 的 i-Monogrid，这是一种含有 M2 血管生成单核细胞的胶原凝胶；对照组 1（N = 5）接受无细胞胶原的应用；对照组 2（N = 5）未接受治疗。皮瓣用订书钉固定到位，并观察伤口缺血和皮瓣皮肤坏死情况。术后 1 周，采集皮肤和下面的肌肉进行组织学分析。
未观察到伤口愈合的宏观差异或皮肤存活的微观差异。然而，单核细胞组的血管改善明显优于 C1（P =.047，chi = 3.96），且与 C2 相比也有改善趋势（P =.103，chi = 2.67）。
将激活的促血管生成单核细胞递送到缺血皮瓣中，倾向于改善血管生成的组织学证据，而没有相应的皮瓣存活的微观或宏观证据。这些结果令人鼓舞，表明单核细胞作为改善缺血组织血管化的潜在方法具有潜力。
