巴西东北部犬类和蜱虫中埃立克体感染的患病率。

Prevalence of ehrlichial infection among dogs and ticks in Northeastern Brazil.

作者信息

Souza Bárbara Maria Paraná da Silva, Leal Danielle Custódio, Barboza Débora Cristina Portela Medina, Uzêda Rosângela Soares, De Alcântara Adriano Costa, Ferreira Fernando, Labruna Marcelo Bahia

机构信息

Laboratório de Infectologia Veterinária --LIVE, Departamento de Produção Animal, Escola de Medicina Veterinária, Universidade Federal da Bahia – UFBA.

出版信息

Rev Bras Parasitol Vet. 2010 Apr-Jun;19(2):89-93. doi: 10.4322/rbpv.01902004.

DOI:10.4322/rbpv.01902004

PMID:20624344

Abstract

This study investigated the epidemiology of canine ehrlichiosis in Northeastern Brazil, focusing the identification of the Ehrlichia species and vectors involved. Samples were collected from 472 domestic dogs residing in the health districts of Cajazeiras and Itapuã of Salvador city. The average prevalence of antibodies reactive to E. canis by immunofluorescent antibody test (IFAT) (titer ≥ 1:80) was 35.6% (168/472). Blood samples from the E. canis-seropositive animals were tested by nested PCR in order to identify the Ehrlichia species responsible for the infection. Among the seropositives, 58 (34.5%) were found to be PCR-positive for E. canis. Ticks were found in 32 dogs. Nested-PCR analysis showed that 21.9% (7/32) of the Rhipicephalus sanguineus were infected by E. canis. In both dogs and Rhipicephalus sanguineus, nested-PCR for E. ewingii and E. chaffeensis was negative, with no amplification of DNA fragment.

摘要

本研究调查了巴西东北部犬埃立克体病的流行病学，重点是鉴定所涉及的埃立克体菌种和传播媒介。从居住在萨尔瓦多市卡亚热拉斯和伊塔普阿卫生区的472只家犬采集样本。通过免疫荧光抗体试验（IFAT）（滴度≥1:80）检测对犬埃立克体反应性抗体的平均患病率为35.6%（168/472）。对犬埃立克体血清阳性动物的血样进行巢式PCR检测，以鉴定引起感染的埃立克体菌种。在血清阳性动物中，58只（34.5%）被检测为犬埃立克体PCR阳性。在32只犬身上发现了蜱。巢式PCR分析显示，21.9%（7/32）的血红扇头蜱感染了犬埃立克体。在犬和血红扇头蜱中，对尤因埃立克体和查菲埃立克体的巢式PCR均为阴性，未扩增出DNA片段。