Department of Hematology, Shengjing Hospital, China Medical University, 110004, Shenyang, China.
Med Oncol. 2011 Dec;28(4):1584-8. doi: 10.1007/s12032-010-9601-5. Epub 2010 Jul 13.
The present study was undertaken to determine the molecular mechanisms by which embelin induces apoptosis in human leukemia cells. Embelin resulted in loss of cell viability and inhibition of proliferation in a dose- and time-dependent manner, which was largely attributed to apoptosis. Embelin caused depolarization of mitochondrial membrane potential. Western blot analysis showed that the expression of anti-apoptotic proteins X-linked inhibitor of apoptosis (XIAP) was down-regulated by embelin. Embelin induced activation of caspase-9 and embelin-induced apoptosis was prevented by caspase inhibitors. Taken together, these findings suggest that embelin results in human leukemia cells apoptosis through caspase-dependent mechanisms involving down-regulation of XIAP.
本研究旨在确定郁李素诱导人白血病细胞凋亡的分子机制。郁李素呈剂量和时间依赖性地导致细胞活力丧失和增殖抑制，这在很大程度上归因于细胞凋亡。郁李素导致线粒体膜电位去极化。Western blot 分析表明，凋亡抑制蛋白 X 连锁抑制剂（XIAP）的表达被郁李素下调。郁李素诱导 caspase-9 的激活，并且 caspase 抑制剂可阻止郁李素诱导的凋亡。总之，这些发现表明郁李素通过 caspase 依赖性机制导致人白血病细胞凋亡，涉及 XIAP 的下调。
