College of Pharmacy, Woosuk University, Jeonju, South Korea.
Immunopharmacol Immunotoxicol. 2011 Jun;33(2):271-8. doi: 10.3109/08923973.2010.500293. Epub 2010 Jul 13.
In this study, we investigated the effect of the Meoruh wine (MW), a Korean traditional wine made by the fruits of Vitis amurensis Rupr. (Vitaceae) on the mast cell-mediated allergic inflammation and studied the possible mechanism of action. Mast cell-mediated allergic disease is involved in many diseases such as anaphylaxis, rhinitis, asthma, and atopic dermatitis. MW inhibited compound 48/80-induced systemic anaphylaxis and serum histamine release in a dose-dependent manner in mice. MW decreased immunoglobulin E-mediated local allergic reactions, passive cutaneous anaphylaxis. MW dose-dependently reduced histamine release from human mast cells activated by phorbol 12-myristate 13-acetate and calcium ionophore A23187 (PMACI). The inhibitory effect of MW on histamine release was mediated by suppression of intracellular calcium. In addition, MW attenuated the PMACI-stimulated expression of pro-inflammatory cytokines such as tumor necrosis factor-α, interleukin-1β (IL-1β), IL-6, and IL-8 in human mast cells. The inhibitory effect of MW on these pro-inflammatory cytokines was nuclear factor (NF)-κB dependent. Our findings provide evidence that MW inhibits mast cell-derived allergic inflammatory reactions and involvement of calcium, pro-inflammatory cytokines and NF-κB in these effects.
在这项研究中，我们研究了梅鲁酒（MW）对肥大细胞介导的过敏炎症的影响，探讨了其可能的作用机制。肥大细胞介导的过敏疾病涉及许多疾病，如过敏反应、鼻炎、哮喘和特应性皮炎。MW 可抑制化合物 48/80 诱导的小鼠全身过敏反应和血清组胺释放，并呈剂量依赖性。MW 可降低免疫球蛋白 E 介导的局部过敏反应，如被动皮肤过敏反应。MW 可剂量依赖性地减少 Phorbol 12-myristate 13-acetate 和钙离子载体 A23187（PMACI）激活的人肥大细胞释放组胺。MW 对组胺释放的抑制作用是通过抑制细胞内钙来介导的。此外，MW 可减弱 PMACI 刺激的人肥大细胞中促炎细胞因子（如肿瘤坏死因子-α、白细胞介素-1β（IL-1β）、IL-6 和 IL-8）的表达。MW 对这些促炎细胞因子的抑制作用依赖于核因子（NF）-κB。我们的研究结果表明，MW 可抑制肥大细胞来源的过敏炎症反应，并参与钙、促炎细胞因子和 NF-κB 在此过程中的作用。
