Department of Chemistry, Pohang University of Science and Technology, Pohang 790-784, Gyeongbuk, Republic of Korea.
Biosens Bioelectron. 2010 Sep 15;26(1):74-9. doi: 10.1016/j.bios.2010.05.015. Epub 2010 May 31.
We describe a novel medium, a captopril/thiophenol (Capt/TP) mixed self-assembled monolayer (SAM) formed on gold, for selectively pre-concentrating serotonin (5-hydroxytryptamine, 5-HT). The 5-HT molecules were shown to be selectively captured via formation of a strong complex, providing anodic stripping currents upon anodic scan or drastic increases in charge-transfer resistances for a redox probe, Fe(CN)(6)(3-/4-) pair, due to their blocking effects. The 5-HT molecules thus collected were subsequently detected by anodic stripping differential pulse voltammetry (ASDPV) or electrochemical impedance spectroscopy (EIS). The detection limit was 1.2 when detected by EIS and 28 nM with ASDPV. 5-HT was shown to be determined in physiological matrices containing ascorbic acid, dopamine, and other commonly encountered neurotransmitters.
我们描述了一种新型介质，即在金上形成的巯基丙氨酸/硫酚（Capt/TP）混合自组装单层（SAM），用于选择性地预浓缩 5-羟色胺（5-羟基色胺，5-HT）。由于其阻断作用，5-HT 分子通过形成强复合物被选择性捕获，在阳极扫描时提供阳极剥离电流，或大大增加氧化还原探针 Fe(CN)（6）（3-/4-）对的电荷转移电阻。因此，通过阳极剥离差分脉冲伏安法（ASDPV）或电化学阻抗谱（EIS）检测收集的 5-HT 分子。通过 EIS 检测的检测限为 1.2，通过 ASDPV 检测的检测限为 28 nM。在含有抗坏血酸、多巴胺和其他常见神经递质的生理基质中，5-HT 得到了很好的检测。
