Miyoshi N, Hara K, Kimura S, Nakanishi K, Fukuda M
Department of Pathology, Fukui Medical School, Japan.
Photochem Photobiol. 1991 Mar;53(3):415-8. doi: 10.1111/j.1751-1097.1991.tb03650.x.
We determined intracellular free Ca2+ concentration by fluorescence spectroscopy and the time-resolved measurements of 2-[(2-amino-5-methylphenoxy) methyl]-6-methoxy-8-aminoquinoline-N,N,N',N'-tetraacetic acid, tetrapotassium salt (Quin2) incorporated in suspended mouse leukemia L1210 cells. The paper reports the following two points. (1) Various fluorescence spectrum patterns in cell suspensions dissolved with Quin2 acetoxy methylester were compared with those of the complex in buffer solution containing esterase. (2) The fluorescence lifetime of Quin2 bound to Ca2+ was approx. 4.5-11 times longer (10 +/- 1 ns) than that (1.5 +/- 0.5 ns) of Quin2. The fraction of the long lifetime component was plotted against the concentration of CaCl2 in buffer solution. From the results obtained, it was found that approx. 35 nM Ca2+ was contained in each L1210 cell.
我们通过荧光光谱法以及对掺入悬浮小鼠白血病L1210细胞中的2-[(2-氨基-5-甲基苯氧基)甲基]-6-甲氧基-8-氨基喹啉-N,N,N',N'-四乙酸四钾盐（喹啉-2，Quin2）进行时间分辨测量，来测定细胞内游离钙离子浓度。本文报道了以下两点。（1）将溶解有Quin2乙酰氧基甲酯的细胞悬液中的各种荧光光谱模式，与含有酯酶的缓冲溶液中该复合物的荧光光谱模式进行了比较。（2）与钙离子结合的Quin2的荧光寿命约为Quin2本身荧光寿命（1.5±0.5纳秒）的4.5 - 11倍（10±1纳秒）。将长寿命成分的比例相对于缓冲溶液中氯化钙的浓度作图。从所得结果发现，每个L1210细胞中约含有35纳摩尔钙离子。
