Brand T, Slob A K
Department of Endocrinology and Reproduction, Faculty of Medicine and Health Sciences, Erasmus University, Rotterdam, The Netherlands.
Physiol Behav. 1991 Mar;49(3):549-55. doi: 10.1016/0031-9384(91)90279-w.
The possible prenatal organizing effects of testosterone (T) on adult sexual partner preference, i.e., sexual orientation in female rats, were studied through prenatal exposure (days 11-22) of female fetuses to the antiandrogens flutamide (Sch 13521; 4'-nitro-3'-trifluoromethylisobutyranilide; 5 or 10 mg/day; Experiment 1) or anandron [RU 23908; 5,5-dimethyl-3-(4-nitro-3-(trifluoromethyl)phenyl)- 2,4-imidazolidinedione; 35 mg/kg/day; Experiment 2]. The neonatal organizing effects of T were further studied by giving T, dihydrotestosterone (DHT) or oil within 9 h after birth to female pups (Experiment 3). In adulthood sexual orientation was ascertained, after ovariectomy followed by hormone treatment, in an automated open field (AOF), with stimulus animals behind wire mesh, and in a 3-compartment box (3-CB), with stimulus animals tethered. When given the choice between an estrous female and a sexually active male in the AOF, flutamide females, as well as controls, preferred the male partner. After long-term T treatment and 3 weekly pair-tests with an estrous female, flutamide females as well as controls switched their preference to the estrous female partner. In anadron females similar results were obtained. Thus the prenatal antiandrogens had no significant effect on sexual orientation in female rats. This suggests that adult sexual orientation in female rats is not organized prenatally through endogenous T. The change in preference after sexual experience corroborates earlier findings from our laboratory. When given the choice between an estrous female and a sexually active male in the 3-CB (sexual interaction with incentives possible), neonatally DHTP-treated females preferred the male; neonatally TP- or oil-treated females showed no preference.(ABSTRACT TRUNCATED AT 250 WORDS)
通过在雌性胎儿孕期（第11 - 22天）使其暴露于抗雄激素药物氟他胺（Sch 13521；4'-硝基-3'-三氟甲基异丁酰苯胺；5或10毫克/天；实验1）或阿那雄酮[RU 23908；5,5 - 二甲基-3-(4 - 硝基-3-(三氟甲基)phenyl)-2,4 - 咪唑烷二酮；35毫克/千克/天；实验2]，研究了睾酮（T）对成年雌性大鼠性伴侣偏好即性取向可能的产前组织化作用。通过在出生后9小时内给雌性幼崽注射T、双氢睾酮（DHT）或油，进一步研究了T的新生儿组织化作用（实验3）。成年后，在卵巢切除并进行激素处理后，在自动旷场（AOF）中确定性取向，刺激动物置于金属丝网后，以及在三室箱（3 - CB）中，刺激动物被拴住。当在AOF中在发情雌性和性活跃雄性之间进行选择时，氟他胺处理的雌性以及对照组都更喜欢雄性伴侣。在长期T处理并与发情雌性进行3周每周一次的配对测试后，氟他胺处理的雌性以及对照组将偏好转向了发情雌性伴侣。在阿那雄酮处理的雌性中也得到了类似结果。因此，产前抗雄激素药物对雌性大鼠的性取向没有显著影响。这表明雌性大鼠的成年性取向并非通过内源性T在产前进行组织化。性经验后偏好的改变证实了我们实验室早期的发现。当在3 - CB中在发情雌性和性活跃雄性之间进行选择时（可能存在带有刺激因素的性互动），新生期经DHTP处理的雌性更喜欢雄性；新生期经TP或油处理的雌性没有表现出偏好。（摘要截取自250词）
