[基于磁珠样本分级分离和基质辅助激光解吸电离飞行时间质谱法检测肺腺癌患者血清蛋白]
[Detection of serum proteins in the patients of lung adenocarcinoma by the method of magnetic bead based sample fractionation and MALDI-TOF-MS].
作者信息
Liu Dan, Liu Lun-Xu, Yuan Quan, Li Xiao-Liang, Huang Na, Yang Xiao-Dong
机构信息
Department of Respiratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 May;41(3):498-500, 556.
OBJECTIVE
To screen the serum proteins related to human lung adenocarcinoma using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) technology.
METHODS
The blood samples were collected from 10 patients of lung adenocarcinoma before and one week after the surgery, while 10 healthy subjects were used as control. The differential protein expression between the two groups and the change of those proteins after surgery were studied by ClinProt magnetic bead enrichment and MALDI-TOF-MS.
RESULTS
Six protein peaks were identified, 2 of them were highly expressed protein biomarkers with relative molecular weights of 2661, 2991, and increased after the surgery, 4 of them were lowly expressed protein biomarkers with relative molecular weights of 4091, 4210, 4644, 5336, which continuously decreased after the surgery.
CONCLUSION
ClinProt magnetic bead enrichment and MALDI-TOF-MS is a quick, easy and sensitive method of proteomics. The differential expressed proteins may be the latent tumor marker of lung adenocarcinoma. The alteration of those proteins after surgery might be helpful to assess the therapeutic effect and prognosis.
目的
运用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）技术筛选与人类肺腺癌相关的血清蛋白。
方法
收集10例肺腺癌患者手术前及手术后1周的血样，同时选取10名健康受试者作为对照。采用ClinProt磁珠富集和MALDI-TOF-MS技术研究两组之间的差异蛋白表达及术后这些蛋白的变化。
结果
鉴定出6个蛋白峰，其中2个为高表达蛋白生物标志物，相对分子质量分别为2661、2991，术后升高；4个为低表达蛋白生物标志物，相对分子质量分别为4091、4210、4644、5336，术后持续下降。
结论
ClinProt磁珠富集和MALDI-TOF-MS是一种快速、简便且灵敏的蛋白质组学方法。差异表达蛋白可能是肺腺癌的潜在肿瘤标志物。这些蛋白术后的变化可能有助于评估治疗效果和预后。
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