Department of Clinical Sciences, Cornell University, Ithaca, NY 14853, USA.
J Dairy Sci. 2010 Jul;93(7):2926-37. doi: 10.3168/jds.2009-2551.
The objective of this study was to evaluate the association between uterine disease and indicators of neutrophil (PMN) and systemic energy status in dairy cows. Peripheral blood (120 mL) was collected weekly from 84 Holstein cows for PMN isolation and plasma collection from calving until 42 d in milk (DIM). The final analysis included 80 cows. Of those, 20 cows were classified as having metritis (fetid uterine discharge and fever), 15 as having subclinical endometritis (SCE; >or=10% PMN on uterine cytology), and 45 as healthy controls. Plasma haptoglobin concentration was increased only in cows that developed metritis. Neutrophil glycogen content was reduced in cows developing metritis compared with healthy cows on the day of calving and at 7 and 42 DIM. Cows with SCE had lower PMN glycogen content than healthy cows at 7, 28, and 42 DIM. Blood glucose was affected by disease status within parity. Primiparous metritis cows had greater blood glucose concentrations than healthy primiparous cows. Multiparous metritis cows tended to have lower blood glucose concentration than multiparous SCE cows. Cows that developed metritis and SCE had or tended to have greater NEFA and BHBA than healthy cows, mainly around calving. At calving, cows that developed metritis had higher plasma estradiol concentration than healthy cows and greater plasma cortisol than cows that had SCE. Plasma insulin was not affected. Plasma glucagon was increased for SCE cows. Cows that developed uterine disease experienced a greater degree of negative energy balance and had decreased lower intracellular PMN glycogen levels, which could be a major predisposing factor for disease because of decreased availability of oxidative fuels.
本研究旨在评估奶牛子宫疾病与中性粒细胞（PMN）和全身能量状态指标之间的关系。从分娩到产后 42 天（DIM），每周从 84 头荷斯坦奶牛采集外周血（120mL）以分离PMN 和采集血浆。最终分析包括 80 头奶牛。其中，20 头奶牛被归类为患有子宫炎（有臭味的子宫分泌物和发热），15 头患有亚临床子宫内膜炎（SCE；子宫细胞学检查中PMN 计数≥10%），45 头为健康对照组。只有患有子宫炎的奶牛血浆触珠蛋白浓度增加。与健康奶牛相比，分娩当天以及产后 7 天和 42 天，患有子宫炎的奶牛PMN 糖原含量降低。患有 SCE 的奶牛与健康奶牛相比，PMN 糖原含量在产后 7、28 和 42 天均较低。血糖受胎次内疾病状况的影响。初产子宫炎奶牛的血糖浓度高于健康初产奶牛。经产子宫炎奶牛的血糖浓度往往低于经产 SCE 奶牛。患有子宫炎和 SCE 的奶牛的 NEFA 和 BHBA 高于健康奶牛，主要是在分娩前后。分娩时，患有子宫炎的奶牛的血浆雌二醇浓度高于健康奶牛，而皮质醇浓度高于患有 SCE 的奶牛。血浆胰岛素不受影响。SCE 奶牛的血浆胰高血糖素增加。患有子宫疾病的奶牛经历了更大程度的负能量平衡，PMN 细胞内糖原水平降低，这可能是疾病的主要易患因素，因为氧化燃料的可用性降低。
