Hammon D S, Evjen I M, Dhiman T R, Goff J P, Walters J L
Department of Animal Dairy and Veterinary Science, Utah State University, Logan, UT 84321, USA.
Vet Immunol Immunopathol. 2006 Sep 15;113(1-2):21-9. doi: 10.1016/j.vetimm.2006.03.022. Epub 2006 Jun 5.
The objectives of this study were to investigate the associations between peripheral blood neutrophil (PMN) function, energy status, and uterine health in periparturient dairy cows. Data were collected from 83 multiparous Holstein cows. Blood samples for PMN function determination were collected weekly from 1 week prior to calving (week -1) through 4 weeks after calving and again at 8 weeks after calving. Energy metabolites were measured and dry matter intake (DMI) was determined from weeks -2 to 5 to evaluate energy status of cows during the periparturient period. All cows were examined for uterine health disorders. Blood PMN killing ability was evaluated by determining myeloperoxidase activity and cytochrome c reduction activity in isolated blood PMN's. For cows that were diagnosed with puerperal metritis and subclinical (SC) endometritis and puerperal metritis, blood PMN functions were significantly (P<0.05) impaired during the periparturient period, compared to cows with normal uterine health. Cows with subclinical endometritis and puerperal metritis or SC endometritis also had significantly (P<0.01) higher NEFA and significantly (P<0.001) lower DMI during the periparturient period, and significantly (P<0.05) higher BHBA during early lactation, compared to cows with normal uterine health. Neutrophil function was also significantly (P<0.01) impaired in cows with peripartum negative energy balance, which was characterized by elevated blood levels of NEFA and decreased DMI. Decreased PMN function and energy balance were associated with uterine health disorders and the decreases in PMN function and energy balance occurred prior to parturition and prior to the detection of these uterine disorders.
本研究的目的是调查围产期奶牛外周血中性粒细胞（PMN）功能、能量状态与子宫健康之间的关联。收集了83头经产荷斯坦奶牛的数据。在产犊前1周（-1周）至产犊后4周每周采集用于PMN功能测定的血样，并在产犊后8周再次采集。测定能量代谢物，并在-2至5周测定干物质摄入量（DMI），以评估奶牛围产期的能量状态。对所有奶牛进行子宫健康障碍检查。通过测定分离的血液PMN中的髓过氧化物酶活性和细胞色素c还原活性来评估血液PMN的杀伤能力。与子宫健康正常的奶牛相比，被诊断为产后子宫炎和亚临床（SC）子宫内膜炎以及产后子宫炎的奶牛，其围产期血液PMN功能显著（P<0.05）受损。与子宫健康正常的奶牛相比，患有亚临床子宫内膜炎和产后子宫炎或SC子宫内膜炎的奶牛在围产期也有显著（P<0.01）更高的非酯化脂肪酸（NEFA）和显著（P<0.001）更低的DMI，并且在泌乳早期有显著（P<0.05）更高的β-羟基丁酸（BHBA）。围产期负能量平衡的奶牛中性粒细胞功能也显著（P<0.01）受损，其特征是血液NEFA水平升高和DMI降低。PMN功能降低和能量平衡与子宫健康障碍有关，并且PMN功能和能量平衡的降低发生在分娩前以及这些子宫疾病被检测到之前。
