Experimental and Clinical Pharmacology Unit, Molecular Oncology and Translational Medicine, Domert, Centro di Riferimento Oncologico, IRCCS National Cancer Institute, via F. Gallini 2, Aviano, PN, Italy.
Dig Dis Sci. 2010 Nov;55(11):3041-6. doi: 10.1007/s10620-010-1323-1. Epub 2010 Jul 15.
Proteomic technologies are being used with increasing frequency in the scientific community. In this review, we have highlighted their use in celiac disease (CD). The available techniques, which include two-dimensional (2D) gel electrophoresis, mass spectrometry, and antibody and tissue arrays, have been used to identify proteins or changes in protein expression specific to gut tissue from patients with CD. A number of studies have employed proteomic methodologies to determine the diagnostic biomarkers in body fluids or to examine changes in protein expression and posttranslational modifications during signaling. A fast technological development of these methods, along with the combination of classic techniques with proteomics, will lead to new discoveries, which will consent a better understanding of the pathogenesis of CD.
蛋白质组学技术在科学界中的应用越来越频繁。在这篇综述中，我们强调了它们在乳糜泻（CD）中的应用。现有的技术包括二维（2D）凝胶电泳、质谱和抗体及组织阵列，这些技术被用于鉴定来自 CD 患者肠道组织的特异性蛋白或蛋白表达变化。许多研究采用蛋白质组学方法来确定体液中的诊断生物标志物，或检查信号转导过程中蛋白表达和翻译后修饰的变化。这些方法的快速技术发展，以及经典技术与蛋白质组学的结合，将带来新的发现，这将有助于更好地理解 CD 的发病机制。
