水杨酸诱导豌豆叶片蛋白的蛋白质组学分析。
Proteomic analysis of salicylate-induced proteins of pea (Pisum sativum L.) leaves.
机构信息
Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, Kazan, 420111, Russia.
出版信息
Biochemistry (Mosc). 2010 May;75(5):590-7. doi: 10.1134/s0006297910050081.
The effect of 50 microM salicylic acid on soluble proteins of pea (Pisum sativum L.) leaves was studied by proteomic analysis. Thirty-two salicylate-induced proteins were found, and 13 of these were identified using MALDI TOF MS. Salicylate-induced increased content was shown for the first time for the family 18 glycoside hydrolase, alpha-amylase, 33 kDa protein of photosystem II, lipid-desaturase-like protein, and glutamine amidotransferase. Increased content of protective proteins of direct antipathogenic action such as chitinase and beta-1,3-glucanases was also noted.
采用蛋白质组学分析方法研究了 50μM 水杨酸对豌豆（Pisum sativum L.）叶片可溶性蛋白的影响。发现了 32 种水杨酸诱导蛋白，其中 13 种用 MALDI-TOF MS 进行了鉴定。首次显示了家族 18 糖苷水解酶、α-淀粉酶、光系统 II 的 33kDa 蛋白、脂脱氢酶样蛋白和谷氨酰胺酰胺转移酶的含量增加。还注意到具有直接抗病原作用的保护蛋白如几丁质酶和β-1,3-葡聚糖酶的含量增加。
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