Plant Biology Laboratory, Drug Development/Diagnostics and Biotechnology Division, CSIRIndian Institute of Chemical Biology, Kolkata 700 032, India.
J Biosci. 2013 Jun;38(2):317-28. doi: 10.1007/s12038-013-9308-9.
Salicylic acid (SA) has been implicated in determining the outcome of interactions between many plants and their pathogens. Global changes in response to this phytohormone have been observed at the transcript level, but little is known of how it induces changes in protein abundance. To this end we have investigated the effect of 1 mM SA on soluble proteins of Arabidopsis thaliana leaves by proteomic analysis. An initial study at transcript level has been performed on temporal landscape, which revealed that induction of most of the SA-responsive genes occurs within 3 to 6 h post treatment (HPT) and the expression peaked within 24 HPT. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF MS/MS analysis has been used to identify differentially expressed proteins and 63 spots have been identified successfully. This comparative proteomic profiling of SA treated leaves versus control leaves demonstrated the changes of many defence related proteins like pathogenesis related protein 10a (PR10a), diseaseresistance- like protein, putative late blight-resistance protein, WRKY4, MYB4, etc. along with gross increase in the rate of energy production, while other general metabolism rate is slightly toned down, presumably signifying a transition from 'normal mode' to 'defence mode'.
水杨酸（SA）被认为在决定许多植物与其病原体相互作用的结果中起关键作用。在转录水平上已经观察到对这种植物激素的全球变化，但对于它如何诱导蛋白质丰度的变化知之甚少。为此，我们通过蛋白质组学分析研究了 1mM SA 对拟南芥叶片可溶性蛋白的影响。在时间景观上进行了初步的转录水平研究，结果表明，大多数 SA 应答基因的诱导发生在处理后 3 到 6 小时（HPT）内，表达峰值出现在 24 HPT 内。二维凝胶电泳（2-DE）结合 MALDI-TOF MS/MS 分析已用于鉴定差异表达蛋白，成功鉴定了 63 个斑点。与对照叶片相比，SA 处理叶片的比较蛋白质组学分析表明，许多防御相关蛋白的变化，如病程相关蛋白 10a（PR10a）、抗病蛋白、假定的晚疫病抗性蛋白、WRKY4、MYB4 等，同时伴随着能量产生速率的大幅增加，而其他一般代谢速率略有下降，这可能表明从“正常模式”到“防御模式”的转变。
