Tatarskiĭ A R, Emirova A S, Bobkov E V, Chuchalin A G
Ter Arkh. 1991;63(3):90-3.
It was shown that platelets of asthmatic patients notwithstanding the disease form are more sensitive to the stimulating action of platelet-activating factor (PAF) in aggregation response and intracellular Ca2+ influx induction than platelets of healthy donors. Intracellular Ca2+ influx was measured using the fluorescent probe Fura-2. Platelet apheresis was applied attempting to normalize the "hyperactivated" state of asthmatic patients' platelets. Platelet apheresis was performed using the on-line cell separator Fenwal CS-3000 in 27 patients without any considerable side effects. The mean platelet yield was 400-600 X 10(9) cells; the blood platelet count restored immediately after the treatment. Positive clinical effect (complete reduction of asthmatic attacks for at least 2 months, improvement in respiratory function parameters) was observed in 19 out of the 27 patients (70%). The clinical effect correlated well with the normalization of in-vitro platelet response to PAF.
结果显示，无论哮喘患者的疾病类型如何，其血小板在聚集反应和诱导细胞内Ca2+内流方面比健康供体的血小板对血小板活化因子（PAF）的刺激作用更敏感。使用荧光探针Fura-2测量细胞内Ca2+内流。采用血小板单采术试图使哮喘患者血小板的“过度活化”状态恢复正常。使用Fenwal CS-3000在线细胞分离器对27例患者进行血小板单采术，未出现任何明显副作用。平均血小板采集量为400 - 600×10(9)个细胞；治疗后血小板计数立即恢复。27例患者中有19例（70%）观察到了积极的临床效果（哮喘发作完全减少至少2个月，呼吸功能参数改善）。临床效果与体外血小板对PAF反应的正常化密切相关。
