Kono H, Chin Lin Y, Gu Y, Yamaguchi M, Zuspan F P, Furuhashi N, Takayama K, Yajima A
Department of Veterinary Physiology and Pharmacology, Ohio State University, Columbus.
Tohoku J Exp Med. 1991 Mar;163(3):149-56. doi: 10.1620/tjem.163.149.
In order to study the effect of gossypol on the monoamine oxidase (MAO) activity in pregnant rat organs, 20 day pregnant rats were sacrificed, and MAO activity/0.02 g tissue of several organs and total MAO activity/organ, were determined. The control group (n = 5) were injected with vehicle intramuscularly on the 17th, 18th and 19th day of pregnancy, and the gossypol treated group (n = 4) were injected with gossypol acetic acid (GAA) (25 mg/kg of body weight) intramuscularly on the 17th, 18th and 19th day and were decapitated on the 20th day of pregnancy. Maternal liver, uterus, placenta, and fetal liver were collected and examined. In placenta, total MAO activity/organ was significantly (p less than 0.05) lower in the GAA treated group than that in the control group. However, in maternal liver, uterus and fetal liver, there was no significant difference in total MAO activity/organ between the two groups. There was no significant difference in MAO activity/0.02 g tissue (specific activity), weight of organ, protein weight/organ and protein/g tissue between the two groups in maternal liver, uterus, placenta and fetal liver. These results suggest that, after intramuscular injection of GAA, total MAO activity/organ in the placenta of pregnant rats might decrease significantly.
为研究棉酚对妊娠大鼠器官中单胺氧化酶（MAO）活性的影响，处死妊娠20天的大鼠，测定多个器官的MAO活性/0.02 g组织以及每个器官的总MAO活性。对照组（n = 5）在妊娠第17、18和19天肌肉注射赋形剂，棉酚处理组（n = 4）在妊娠第17、18和19天肌肉注射醋酸棉酚（GAA）（25 mg/kg体重），并在妊娠第20天断头处死。收集母体的肝脏、子宫、胎盘以及胎儿的肝脏并进行检测。在胎盘中，GAA处理组每个器官的总MAO活性显著低于对照组（p < 0.05）。然而，在母体肝脏、子宫和胎儿肝脏中，两组每个器官的总MAO活性没有显著差异。在母体肝脏、子宫、胎盘和胎儿肝脏中，两组之间的MAO活性/0.02 g组织（比活性）、器官重量、每个器官的蛋白重量以及每克组织的蛋白含量均无显著差异。这些结果表明，肌肉注射GAA后，妊娠大鼠胎盘的每个器官总MAO活性可能会显著降低。
