Kono H, Lin Y C, Yamaguchi M, Zuspan F P, O'Shaughnessy R W, Lee A C, Furuhashi N, Yokaichiya T, Takayama K, Yajima A
Department of Veterinary Physiology and Pharmacology, Ohio State University, Columbus.
Tohoku J Exp Med. 1994 Jan;172(1):1-8. doi: 10.1620/tjem.172.1.
To elucidate the involvement of MAO activity in rat pregnancy, female rats exhibiting at least 3 consecutive 4-day estrous cycles were used. The day of sperm-positive smears was designated as day 0 of pregnancy. The rats were sacrificed on day 2 of diestrus (diestrus-2) and days 7, 14 and 21 of gestation. The organs were collected and weighted, and mitochondrial fractions were prepared for MAO analysis with kynuramine dihydrobromide as substrate. Specific activity (pmol/mg of protein/min) in the ovary is significantly lower in rats on diestrus-2 (792 +/- 127) (mean +/- S.D.) than on day 14 (1,038 +/- 140) and day 21 (1,580 +/- 61) of pregnancy. However, the levels of MAO activity in the uterus of rats on diestrus-2 (899 +/- 266) is higher than on day 21 (570 +/- 80) of pregnancy. The MAO activity in the placenta on day 21 (644 +/- 82) was higher than day 14 (356 +/- 88) of pregnancy. Total MAO activity per organ (nmol/min) of ovary on day 21 (27.2 +/- 5.2) is higher than on diestrus-2 (8.7 +/- 3.0) and on day 14 (14.2 +/- 3.6). Our results suggest that MAO activity in rat reproductive organs such as uterus, ovary, and placenta might fluctuate significantly near term, in correlation with steroid levels, tissue catecholamine contents and so on, in order to maintain pregnancy.
为阐明单胺氧化酶（MAO）活性在大鼠妊娠中的作用，选用了至少有3个连续4天发情周期的雌性大鼠。精子涂片呈阳性的当天被定为妊娠第0天。在动情后期第2天（动情后期-2）以及妊娠第7、14和21天处死大鼠。收集器官并称重，制备线粒体部分，以二氢溴化犬尿胺为底物进行MAO分析。动情后期-2的大鼠卵巢中的比活性（pmol/毫克蛋白/分钟）（792±127）（平均值±标准差）显著低于妊娠第14天（1038±140）和第21天（1580±61）。然而，动情后期-2的大鼠子宫中的MAO活性水平（899±266）高于妊娠第21天（570±80）。妊娠第21天胎盘的MAO活性（644±82）高于妊娠第14天（356±88）。妊娠第21天卵巢的每个器官的总MAO活性（nmol/分钟）（27.2±5.2）高于动情后期-2（8.7±3.0）和第14天（14.2±3.6）。我们的结果表明，大鼠生殖器官如子宫、卵巢和胎盘的MAO活性在妊娠末期可能会显著波动，这与类固醇水平、组织儿茶酚胺含量等相关，以维持妊娠。
