Division of Cardiology, Fletcher Allen Health Care and the University of Vermont School of Medicine, Burlington, VT 05401, USA.
Mediators Inflamm. 2010;2010:250476. doi: 10.1155/2010/250476. Epub 2010 Jun 16.
Endothelial Microparticles (EMPs) are small vesicles shed from activated or apoptotic endothelial cells and involved in cellular cross-talk. Whether EMP immunophenotypes vary according to stimulus in Diabetes Mellitus (DM) is not known. We studied the cellular adhesion molecule (CAM) profile of circulating EMPs in patients with and without Diabetes Mellitus type 2, who were undergoing elective cardiac catheterization.
EMPs were analyzed by flow cytometry. The absolute median number of EMPs (EMPs/microL) specific for CD31, CD105, and CD106 was significantly increased in the DM population. The ratio of CD62E/CD31 EMP populations reflected an apoptotic process.
Circulating CD31+, CD105+, and CD106+ EMPs were significantly elevated in patients with DM. EMPs were the only independent predictors of DM in our study cohort. In addition, the EMP immunophenotype reflected an apoptotic process. Circulating EMPs may provide new options for risk assessment.
内皮细胞微颗粒（EMPs）是从激活或凋亡的内皮细胞脱落的小囊泡，参与细胞间通讯。在糖尿病（DM）中，刺激是否会导致 EMP 免疫表型发生变化尚不清楚。我们研究了接受选择性心导管检查的 2 型糖尿病患者和非糖尿病患者循环 EMPs 的细胞黏附分子（CAM）谱。
通过流式细胞术分析 EMPs。DM 人群中，特定于 CD31、CD105 和 CD106 的 EMPs 的绝对中位数（EMPs/μL）明显增加。CD62E/CD31 EMP 群体的比例反映了凋亡过程。
DM 患者循环 CD31+、CD105+ 和 CD106+ EMPs 显著升高。在我们的研究队列中，EMPs 是 DM 的唯一独立预测因子。此外，EMP 免疫表型反映了凋亡过程。循环 EMPs 可能为风险评估提供新的选择。
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