Department of Pharmacology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia.
Hospital USM, Health Campus, Universiti Sains Malaysia, Kota Bharu 16150, Kelantan, Malaysia.
Medicina (Kaunas). 2022 Jun 19;58(6):824. doi: 10.3390/medicina58060824.
Background and Objectives: Endothelial microparticles (EMP) particularly CD31+/42−/AV+, CD144+/AV+ and CD62e+/AV+ have been reported as having increased in cardiovascular-related diseases, making them potential biomarkers for endothelial dysfunction. This study aimed to compare these EMPs in patients with hypercholesterolemia and healthy controls and to correlate their levels with endothelium-dependent vasodilation (EDV) assessed via pulse wave analysis (PWA); an established method of assessing endothelial function. Materials and Methods: EMPs from 88 subjects (44 hypercholesterolemia patients and 44 controls) were quantified from whole blood using flow cytometry analysis. Endothelial function was determined using PWA combined with pharmacological challenge. Results: CD31+/42−/AV+ (3.45 ± 4.74 count/µL vs. 1.33 ± 4.40 count/µL; p = 0.03), CD144+/AV+ (7.37 ± 12.66 count/µL vs. 1.42 ± 1.71 count/µL; p = 0.003) and CD62e+/AV+ (57.16 ± 56.22 count/µL vs. 20.78 ± 11.04 count/µL; p < 0.001) were significantly elevated in the hypercholesterolemic group compared with the controls, respectively. There was a significant inverse moderate correlation between all circulating EMPs and EDV: CD31+/42−/AV+ (r = −0.36, p = 0.001), CD144+/AV+ (r = −0.37, p = 0.001) and CD62e+/AV+ (r = −0.35, p = 0.002). Conclusions: All EMPs were raised in the patients with hypercholesterolemia, and these values correlated with the established method of assessing endothelial function.
已有研究报道，内皮细胞微颗粒（EMP），特别是 CD31+/42−/AV+、CD144+/AV+ 和 CD62e+/AV+，在心血管相关疾病中增加，使其成为内皮功能障碍的潜在生物标志物。本研究旨在比较高胆固醇血症患者和健康对照组之间这些 EMP 的水平，并将其与通过脉搏波分析（PWA）评估的内皮依赖性血管舒张（EDV）相关联；PWA 是评估内皮功能的一种既定方法。材料和方法：使用流式细胞术分析从 88 名受试者（44 名高胆固醇血症患者和 44 名对照者）的全血中定量 EMP。使用 PWA 结合药物刺激来确定内皮功能。结果：与对照组相比，CD31+/42−/AV+（3.45 ± 4.74 个/µL 比 1.33 ± 4.40 个/µL；p = 0.03）、CD144+/AV+（7.37 ± 12.66 个/µL 比 1.42 ± 1.71 个/µL；p = 0.003）和 CD62e+/AV+（57.16 ± 56.22 个/µL 比 20.78 ± 11.04 个/µL；p < 0.001）在高胆固醇血症组中显著升高。所有循环 EMP 与 EDV 之间存在显著的负中度相关性：CD31+/42−/AV+（r = -0.36，p = 0.001）、CD144+/AV+（r = -0.37，p = 0.001）和 CD62e+/AV+（r = -0.35，p = 0.002）。结论：高胆固醇血症患者的所有 EMP 均升高，这些值与评估内皮功能的既定方法相关。
