C5-6 Technologies and Great Lakes Bioenergy Research Center, Middleton, WI 53511, USA.
Appl Biochem Biotechnol. 2011 Jan;163(2):205-14. doi: 10.1007/s12010-010-9029-6. Epub 2010 Jul 16.
The genome of Dictyoglomus turgidum was sequenced and analyzed for carbohydrases. The broad range of carbohydrate substrate utilization is reflected in the high number of glycosyl hydrolases, 54, and the high percentage of CAZymes present in the genome, 3.09% of its total genes. Screening a random clone library generated from D. turgidum resulted in the discovery of five novel biomass-degrading enzymes with low homology to known molecules. Whole genome sequencing of the organism followed by bioinformatics-directed amplification of selected genes resulted in the recovery of seven additional novel enzyme molecules. Based on the analysis of the genome, D. turgidum does not appear to degrade cellulose using either conventional soluble enzymes or a cellulosomal degradation system. The types and quantities of glycosyl hydrolases and carbohydrate-binding modules present in the genome suggest that D. turgidum degrades cellulose via a mechanism similar to that used by Cytophaga hutchinsonii and Fibrobacter succinogenes.
对 Dictyoglomus turgidum 的基因组进行了测序和分析，以研究其碳水化合物酶。广泛的碳水化合物底物利用反映在大量的糖苷水解酶（54 个）和高比例的 CAZymes 上，占基因组总基因的 3.09%。从 D. turgidum 生成的随机克隆文库的筛选导致发现了五种具有低同源性的新型生物质降解酶。对该生物进行全基因组测序，然后通过生物信息学定向扩增选定的基因，回收了另外七种新型酶分子。基于基因组分析，D. turgidum 似乎既不使用常规可溶性酶也不使用纤维小体降解系统来降解纤维素。基因组中存在的糖苷水解酶和碳水化合物结合模块的类型和数量表明，D. turgidum 通过类似于 Cytophaga hutchinsonii 和 Fibrobacter succinogenes 所使用的机制来降解纤维素。
