Ghimire S R, Saxena A K, Rai D, Dhungel S
Department of Anatomy, Patan Academy of Health Sciences, Lalitpur, Nepal.
Nepal Med Coll J. 2009 Dec;11(4):268-71.
Consumption of alcohol during pregnancy results in fetal alcohol syndrome (FAS) in newborn affecting the central nervous system which is more sensitive to deleterious effect of alcohol. This study was conducted to observe the histological alterations in cerebellum of rat pups born to alcohol consuming mother rats. Virgin female albino rats were given 20.0% (v/v) alcohol through oral route two weeks prior to mating and continued till the weaning of their offspring. On postnatal day 27 (PND27), rat pups were sacrificed. Their brains were collected and weighed. The cerebellums were isolated and processed for histological study. The diameter of Purkinje cell and width of molecular and granular layers of the cerebellar hemisphere were measured. Results showed significantly decreased brain weight in rat pups of experimental group when compared to control. The diameter of Purkinje cells, width of molecular and granular layers were also found to be decreased in the experimental group. These results suggest that the maternal consumption of alcohol affects the brain growth and induces significant alterations in the histological architecture of cerebellum of growing rats.
孕期饮酒会导致新生儿出现胎儿酒精综合征（FAS），影响中枢神经系统，而中枢神经系统对酒精的有害影响更为敏感。本研究旨在观察饮酒的母鼠所产幼鼠小脑的组织学改变。在交配前两周，通过口服途径给未孕雌性白化大鼠给予20.0%（v/v）的酒精，并持续至其后代断奶。在出生后第27天（PND27），处死幼鼠。收集其大脑并称重。分离小脑并进行组织学研究。测量浦肯野细胞的直径以及小脑半球分子层和颗粒层的宽度。结果显示，与对照组相比，实验组幼鼠的脑重显著降低。实验组中浦肯野细胞的直径、分子层和颗粒层的宽度也有所减小。这些结果表明，母鼠饮酒会影响大脑发育，并在生长中大鼠的小脑组织结构中引起显著改变。
